Figure 12.12
800
700
BNC
1:5
***
600
1:10
***
500
***
400
300
200
100
0
% Cell Proliferation
24
48
96
Time (h)
Bacterial Nanocellulose as a Structured Platform for Conductive Biopolymers 257
Figure 12.12
Cell.proliferation.assessed.by.MTS.assay.on.BNC.and.BNC/PAni.HCl.
assay. (Promega,. Madison,. WI),. according. to. the. instructions. provided. by.
the.manufacturer..One.hundred.milliliters.from.each.well.were.transferred.
to.clean.wells.and.the.absorbance.read.at.490.nm..Results.were.expressed.
relatively.to.t.=.0.h,.which.was.considered.as.100%.of.cell.proliferation
As.can.be.observed.(Figure 12.12),.coating.BNC.with.PAni.did.not.result.
in.any.toxic.effect.to.the.cells,.as.none.of.them.resulted.in.a.decrease.of.cell.
viability. to. values. below. the. initial. ones. (considered. as. 100%).. Moreover,.
none.of.the.PAni-treated.BNC.affected.cell.proliferation.up.to.48.h..However,.
at.96.h,.a.statistically.significant.decrease.in.cell.proliferation.was.detected.
for.the.modified.celluloses..These.results.suggest.that.BNC/PAni·HCl.might.
provide.a.novel.conductive.material.well.suited.as.biocompatible.scaffolds.
for.tissue.engineering.
Conclusions
In. today’s. world,. plastic. and. electrical. devices. walk. hand. in. hand..
Since. the. discovery. in. the. late. 1970s. of. an. electrically. conductive,. quasi.
one-dimensional. organic. polymer,. polyacetylene,. a. wide. variety. of. conducting.polymers.have.been.developed..The.high.hopes.raised.by.these.new.
classes.of.polymers.can.easily.be.evidenced.by.the.ever-growing.attraction.
of. researchers. from. diverse. areas. of. interest. such. as. synthetic. chemistry,.
electrochemistry,. solid-state. physics,. materials. science,. polymer. science,.
electronics,.and.electrical.engineering..Given.the.availability.of.methods.to.
800
700
BNC
1:5
***
600
1:10
***
500
***
400
300
200
100
0
% Cell Proliferation
24
48
96
Time (h)
Bacterial Nanocellulose as a Structured Platform for Conductive Biopolymers 257
Figure 12.12
Cell.proliferation.assessed.by.MTS.assay.on.BNC.and.BNC/PAni.HCl.
assay. (Promega,. Madison,. WI),. according. to. the. instructions. provided. by.
the.manufacturer..One.hundred.milliliters.from.each.well.were.transferred.
to.clean.wells.and.the.absorbance.read.at.490.nm..Results.were.expressed.
relatively.to.t.=.0.h,.which.was.considered.as.100%.of.cell.proliferation
As.can.be.observed.(Figure 12.12),.coating.BNC.with.PAni.did.not.result.
in.any.toxic.effect.to.the.cells,.as.none.of.them.resulted.in.a.decrease.of.cell.
viability. to. values. below. the. initial. ones. (considered. as. 100%).. Moreover,.
none.of.the.PAni-treated.BNC.affected.cell.proliferation.up.to.48.h..However,.
at.96.h,.a.statistically.significant.decrease.in.cell.proliferation.was.detected.
for.the.modified.celluloses..These.results.suggest.that.BNC/PAni·HCl.might.
provide.a.novel.conductive.material.well.suited.as.biocompatible.scaffolds.
for.tissue.engineering.
Conclusions
In. today’s. world,. plastic. and. electrical. devices. walk. hand. in. hand..
Since. the. discovery. in. the. late. 1970s. of. an. electrically. conductive,. quasi.
one-dimensional. organic. polymer,. polyacetylene,. a. wide. variety. of. conducting.polymers.have.been.developed..The.high.hopes.raised.by.these.new.
classes.of.polymers.can.easily.be.evidenced.by.the.ever-growing.attraction.
of. researchers. from. diverse. areas. of. interest. such. as. synthetic. chemistry,.
electrochemistry,. solid-state. physics,. materials. science,. polymer. science,.
electronics,.and.electrical.engineering..Given.the.availability.of.methods.to.