Figure 12.11
256
Bacterial Cellulose
10 µm
10 µm
50 µm
50 µm
(d)
(c)
(a)
(b)
Figure 12.11
SEM.micrographs.of.L929,.fibroblast.cells.attached.on.the.surface.of.BNC/PPy.membranes.
therapy.research,.stem.cell.3D.differentiation,.cancer.and.stem.cell.research,.
and.different.cell.type.cultures.and.cocultures.
Compared. to. PPy,. few. groups. have. considered. PAni. as. potential. conductive. substrates. for. tissue. engineering. applications. (Bidez. et. al.. 2006;.
Kamalesh.et.al..2000;.Li.et.al..2006)..In.the.past.2.decades,.the.ability.to.tailor.
the.oxidative.state.of.ICPs.has.caused.the.study.of.PAni.in.a.wide.range.of.
research. fields. such. as. for. biosensors. or. as. scaffolds. in. tissue. engineering.
(Karyakin.et.al..1996;.Wei.et.al..2004)..Figure 12.12.shows.the.cell.proliferation. assays. by. MTS. on. BNC. and. BNC/PAni·HCl.. 3T3. cells. were. cultured.
in. Dulbecco’s. Modified. Eagle’s. Medium. (DMEM). supplemented. with. 10%.
calf. serum. and. 1%. penicillin/streptomycin. and. maintained. at. 37°C. in. a.
humidified.incubator.containing.5%.CO 2 ..They.were.fed.every.2.days.and.
subcultured. once. they. reached. 70%–90%. confluence. by. treatment. with. a.
trypsin/ethylenediaminetetraacetic.acid.(EDTA).solution.
On.the.day.of.the.experiment,.cells.were.seeded.(2.×.10 5 .cells/ml).on.the.
top. of. polyaniline-treated. or. nontreated. bacterial. discs. previously. placed.
on. the. . bottom. of. 96-well. plates.. The. effects. of. the. different. treatments. on.
cell. viability. were. assessed. after. 24,. 48,. and. 96. h. by. using. the. Cell. Titer.
96 . Aqueous. ONE. Solution. Reagent. (MTS. [3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethoxyphenyl)-2-(4-sulfo-phenyl)-2H-tetrazolium]. colorimetric.
256
Bacterial Cellulose
10 µm
10 µm
50 µm
50 µm
(d)
(c)
(a)
(b)
Figure 12.11
SEM.micrographs.of.L929,.fibroblast.cells.attached.on.the.surface.of.BNC/PPy.membranes.
therapy.research,.stem.cell.3D.differentiation,.cancer.and.stem.cell.research,.
and.different.cell.type.cultures.and.cocultures.
Compared. to. PPy,. few. groups. have. considered. PAni. as. potential. conductive. substrates. for. tissue. engineering. applications. (Bidez. et. al.. 2006;.
Kamalesh.et.al..2000;.Li.et.al..2006)..In.the.past.2.decades,.the.ability.to.tailor.
the.oxidative.state.of.ICPs.has.caused.the.study.of.PAni.in.a.wide.range.of.
research. fields. such. as. for. biosensors. or. as. scaffolds. in. tissue. engineering.
(Karyakin.et.al..1996;.Wei.et.al..2004)..Figure 12.12.shows.the.cell.proliferation. assays. by. MTS. on. BNC. and. BNC/PAni·HCl.. 3T3. cells. were. cultured.
in. Dulbecco’s. Modified. Eagle’s. Medium. (DMEM). supplemented. with. 10%.
calf. serum. and. 1%. penicillin/streptomycin. and. maintained. at. 37°C. in. a.
humidified.incubator.containing.5%.CO 2 ..They.were.fed.every.2.days.and.
subcultured. once. they. reached. 70%–90%. confluence. by. treatment. with. a.
trypsin/ethylenediaminetetraacetic.acid.(EDTA).solution.
On.the.day.of.the.experiment,.cells.were.seeded.(2.×.10 5 .cells/ml).on.the.
top. of. polyaniline-treated. or. nontreated. bacterial. discs. previously. placed.
on. the. . bottom. of. 96-well. plates.. The. effects. of. the. different. treatments. on.
cell. viability. were. assessed. after. 24,. 48,. and. 96. h. by. using. the. Cell. Titer.
96 . Aqueous. ONE. Solution. Reagent. (MTS. [3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethoxyphenyl)-2-(4-sulfo-phenyl)-2H-tetrazolium]. colorimetric.