Figure 5.3
95
Bacterial Cellulose Surface Modifications
Column I
Column II
Column III
Column IV
BCbare
BCRGD
ePTFEbare
ePTFE
BCCBM
BC
Figure 5.3
SEM.images.of.the.adhered.platelets.on.endothelialized.BC.untreated.or.treated.with.recombinant.proteins.and.expanded.polytetrafluoroethylene.(ePTFE)..The.bare.BC.and.ePTFE.were.
used.as.controls..The.captions.without.‘‘bare’’.indicate.the.cultured.HMEC.surface..Columns II.
and. IV. (scale. bar. 10. μm). are. the. magnified. images. of. columns. I. and. II. (scale. bar. 30. μm),.
. respectively..(Reproduced.from.Andrade.et.al..2011.)
(CM-BNC). were. produced.. No. morphological. changes. were. observed. in.
either.kind.of.membrane.produced..The.study.showed.that.L929.fibroblast.
cells.grew.better.on.the.modified.membranes.in.comparison.with.the.native.
ones,. with. TMAHP-BNC. membrane. being. the. most. suitable. scaffold. for.
cell. growth.. Further,. the. TMAHP-BNC. was. coated. with. adhesive. protein.
(. collagen.type.I).and.tested.as.a.support.for.the.production.of.recombinant.
neered.Chinese.hamster.ovary.cells..Both.the.viability.of.the.cells.and.production.of.EDF/activin.A.were.maintained.for.about.1.month,.while.cultures.
on.plastic.dishes.lasted.only.12.days.
Trauma.and.disease.of.bone.and.joints,.frequently.damaging.both.the.articular.cartilage.and.the.subchondral.bone,.result.in.severe.pain.and.disability.
95
Bacterial Cellulose Surface Modifications
Column I
Column II
Column III
Column IV
BCbare
BCRGD
ePTFEbare
ePTFE
BCCBM
BC
Figure 5.3
SEM.images.of.the.adhered.platelets.on.endothelialized.BC.untreated.or.treated.with.recombinant.proteins.and.expanded.polytetrafluoroethylene.(ePTFE)..The.bare.BC.and.ePTFE.were.
used.as.controls..The.captions.without.‘‘bare’’.indicate.the.cultured.HMEC.surface..Columns II.
and. IV. (scale. bar. 10. μm). are. the. magnified. images. of. columns. I. and. II. (scale. bar. 30. μm),.
. respectively..(Reproduced.from.Andrade.et.al..2011.)
(CM-BNC). were. produced.. No. morphological. changes. were. observed. in.
either.kind.of.membrane.produced..The.study.showed.that.L929.fibroblast.
cells.grew.better.on.the.modified.membranes.in.comparison.with.the.native.
ones,. with. TMAHP-BNC. membrane. being. the. most. suitable. scaffold. for.
cell. growth.. Further,. the. TMAHP-BNC. was. coated. with. adhesive. protein.
(. collagen.type.I).and.tested.as.a.support.for.the.production.of.recombinant.
neered.Chinese.hamster.ovary.cells..Both.the.viability.of.the.cells.and.production.of.EDF/activin.A.were.maintained.for.about.1.month,.while.cultures.
on.plastic.dishes.lasted.only.12.days.
Trauma.and.disease.of.bone.and.joints,.frequently.damaging.both.the.articular.cartilage.and.the.subchondral.bone,.result.in.severe.pain.and.disability.