Figure 5.2
 
94
Bacterial Cellulose
1 hour
(a)
(b)
(c)
(d)
(e)
(f )
(g)
5 hours
24 hours
48 hours
Figure 5.2
Photographs. showing.the.effect.of.the.recombinant. proteins.on.cell.(fibroblasts. 3T3).attachment.to.polystyrene.plate..(a).RGD/CBM,.(b).RGD/CBM/RGD,.(c).GRGDY/CBM,.(d).GRGDY/
CBM/GRGDY,.(e).CBM,.(f).buffer,.and.(g).control..The.photographs.were.taken.at.1,.5,.24,.and.
48.h.after.the.addition.of.cells..(Reproduced.from.Andrade.et.al..2009.)
limitation.. Therefore,. in. the. mentioned. work,. heparin. was. combined. with.
BNC. during. the. growth. of. Gluconacetobacter xylinus. by. adding. heparin. to.
the.culture.medium..Results.revealed.no.differences.in.morphology.between.
Hep-BNC.and.BNC,.although.significant.changes.were.observed.in.the.crystallographic.structure.when.heparin.was.incorporated.into.the.BNC’s.flask.
cultures.. In. addition,. Fourier. transform. infrared. spectroscopy. (FTIR). and.
X-ray.photoelectron.spectroscopy.(XPS).analysis.demonstrated.the.presence.
of. the. anticoagulant. sulfate. groups. in. the. Hep-BC. hybrid. nanofibers,. suggesting.their.potential.use.for.blood-related.products.
In.order.to.improve.cell.adhesion,.Watanabe.et.al..(1993).chemically.modified.
BNC,.aiming.the.introduction.of.electric.charge..In.this.context,.membranes.of.
trimethyl.ammonium.betahydroxy.propyl-BC.(TMAHP-BNC),.diethyl.aminoethyl-BNC. (DEAE-BNC),. aminoethyl-BC. (AE-BNC),. and. carboxymethyl-BC.