Advances in Genome Editing Tools
213
TABLE 14.1 (Continued)
TABLE 14.1 (Continued)
Gene(s)
Year Species F0/F1
Reference/Notes
Gene(s)
Year Species F0/F1
Reference/Notes
tyr, pax6
2015 laevis
F0
( Miyamoto et al., 2015 )
tyr
2017 tropicalis F0
( Park et al., 2017 )
oocyte host transfer
base editing
cygb
2015 laevis
F0
( Nakade et al., 2015 )
npffr1.1
2017 laevis
F0
( Waqas et al., 2017 )
pax6
2015 tropicalis F1
( Nakayama et al., 2015 )
lhx1, slc45a2
2018 laevis
F0
( DeLay et al., 2018 )
compound het
thrb
2018 tropicalis F0
( Sakane et al., 2018 )
apc
2015 tropicalis F0
( Nieuwenhuysen et al., 2015 )
fgfr4
2018 tropicalis F0
( Sempou et al., 2018 )
thra
2015 tropicalis F0
( Wen and Shi, 2015 )
tbx5
2018 tropicalis F0
( Steimle et al., 2018 )
dot1l
2015 tropicalis F0
( Wen et al., 2015 )
rnf43, znrf3, rspo2 2018 tropicalis F0
( Szenker-Ravi et al., 2018 )
ouro1, ouro2,
2016 tropicalis F0
( Nakai et al., 2016 )
supernumery limbs, linked to
foxn1
clinic
thra
2017 tropicalis F2
( Choi et al., 2017 )
katnal2
2018 tropicalis F0
( Willsey et al., 2018 )
hps6
2017 tropicalis F1
( Nakayama et al., 2017 )
agrees with MO data
agrees with MO data, ENU
xnc10
2019 laevis
( Banach et al., 2019 )
or natural mutation
cell line knockout
p2ry4
2017 laevis
F0
( Harata et al., 2019 )
dsp
2019 laevis
F0
( Bharathan and Dickinson,
mad1
2017 tropicalis F2
( Okada et al., 2017 )
2019 )
tyr
2017 laevis
F1
( Ratzan et al., 2017 )
agrees with MO data
L and S mutants from oocyte
l1cam, crb2
2019 tropicalis F0
( Date et al., 2019 )
host transfer. Compound
dnmbp
2019 laevis
F0
( DeLay et al., 2019 )
hets
MO as well
thra
2017 tropicalis F2
( Wen et al., 2017 )
tbx4
2019 tropicalis F0
( Kariminejad et al., 2019 )
tbxt/tbxt.2
2018 tropicalis F1/F4 ( Gentsch et al., 2018 )
link to clinic, limb defect
F1 mutants for tbxt; tbxt/tbxt2
neurod2
2019 tropicalis F0
( Sega et al., 2019 )
double knockouts in F4
link to clinic; RNA
thra/thrb
2018 tropicalis F2/F1 ( Nakajima et al., 2018 )
overexpression
mecom
2018 tropicalis F2
( Okada and Shi, 2018 )
six1
2019 tropicalis F0
( Sullivan et al., 2019 )
pomc
2020 tropicalis F1
( Shewade et al., 2020 )
rpe65, gnat1
2019 laevis
F0
( Wen et al., 2019 )
compound het
ctnnd1
2020 tropicalis F0
( Alharatani et al., 2020 )
prmt1
2020 tropicalis F2
( Shibata et al., 2019 )
trio
2020 tropicalis F0
( Barbosa et al., 2020 )
thra
2021 tropicalis F2
( Tanizaki et al., 2021 b)
link to clinic, domain specif c
CRISPR
effect
tyr
tyr
cela1.2, ets1, ets2,
hspa5, hhex, pgat,
pdx1, ptf1a,
2013
2013
2014
tropicalis F0/F1 ( Blitz et al., 2013 )
tropicalis F0/F1 ( Nakayama et al., 2013 )
tropicalis F0/F1 ( Guo et al., 2014 )
pax9
tnnc1
dlg5
rb1, rbl1, tp53
2020
2020
2020
2020
tropicalis
tropicalis
tropicalis
tropicalis
F0
F0
F0
F0
( Farley-Barnes et al., 2020 )
( Landim-Vieira et al., 2020 )
(Marquez et al., 2021 )
link to clinic
( Naert et al., 2020a )
tm4sf4, tyr
foxj1, pax8, dnah9,
galnt1, ctnnb1
tubb2b, tyr
ptf1a, tyr
gsc, tyr
cfap299
pax3, snai1,
ctnnb1, sox9,
tfap2a, pax3, zic1,
snai2,
rb1, rbl1
2015
2015
2015
2016
2016
2016
2016
tropicalis F0
( Bhattacharya et al., 2015 )
agrees with MO data
tropicalis F0/F1 ( Shi et al., 2015 )
knockin GFP, ela-GFP
laevis
F0
( Wang et al., 2015 )
agrees with MO data
tropicalis F0/F1 ( Blitz et al., 2016 )
leapfrogging, F0 and F1
phenotypes
laevis
F0
( Jaffe et al., 2016 )
tropicalis F0
( Liu et al., 2016 )
tropicalis F0
( Naert et al., 2016 )
dual knockout
multiple
junB
cfap43, foxj1
daam2
thrb
thra/thrb
adprhl1
ednra, ednrb2,
edn1, edn3
nr3c1
2020
2020
2020
2020
2020
2020
2020
2020
2020
cancer
both
( Naert et al., 2020b )
designing eff cient guides
tropicalis F0/F1 ( Nakamura et al., 2020 )
compound hets
laevis
F0
( Rachev et al., 2020 )
agrees with MO data
tropicalis F0
( Schneider et al., 2020 )
link to clinic
tropicalis F2
( Shibata et al., 2020a )
tropicalis F2
( Shibata et al., 2020b )
double knockout
laevis
F0
( Smith et al., 2020 )
agrees with MO data
laevis
F0
( Square et al., 2020 )
tropicalis F2
( Sterner et al., 2020 )
ctnnb1
2017 both
F0/F1 ( Aslan et al., 2017 )
knock in by oocyte host
transfer
ncoa3
gja8, dnase2b
2020
2020
tropicalis
laevis
F1
F0
( Tanizaki et al., 202 1a)
compound hets
( Viet et al., 2020 )
xnc genes
rho
2017
2017
laevis
laevis
F0
( Banach et al., 2017 )
F0/F1 ( Feehan et al., 2017 )
Msmb.3
mtnr1a
2020
2020
tropicalis F0
( Wang et al., 2020 )
agrees with MO data
tropicalis F0/F1 ( Wiechmann et al., 2020 )
(Continued)
(Continued)
213
TABLE 14.1 (Continued)
TABLE 14.1 (Continued)
Gene(s)
Year Species F0/F1
Reference/Notes
Gene(s)
Year Species F0/F1
Reference/Notes
tyr, pax6
2015 laevis
F0
( Miyamoto et al., 2015 )
tyr
2017 tropicalis F0
( Park et al., 2017 )
oocyte host transfer
base editing
cygb
2015 laevis
F0
( Nakade et al., 2015 )
npffr1.1
2017 laevis
F0
( Waqas et al., 2017 )
pax6
2015 tropicalis F1
( Nakayama et al., 2015 )
lhx1, slc45a2
2018 laevis
F0
( DeLay et al., 2018 )
compound het
thrb
2018 tropicalis F0
( Sakane et al., 2018 )
apc
2015 tropicalis F0
( Nieuwenhuysen et al., 2015 )
fgfr4
2018 tropicalis F0
( Sempou et al., 2018 )
thra
2015 tropicalis F0
( Wen and Shi, 2015 )
tbx5
2018 tropicalis F0
( Steimle et al., 2018 )
dot1l
2015 tropicalis F0
( Wen et al., 2015 )
rnf43, znrf3, rspo2 2018 tropicalis F0
( Szenker-Ravi et al., 2018 )
ouro1, ouro2,
2016 tropicalis F0
( Nakai et al., 2016 )
supernumery limbs, linked to
foxn1
clinic
thra
2017 tropicalis F2
( Choi et al., 2017 )
katnal2
2018 tropicalis F0
( Willsey et al., 2018 )
hps6
2017 tropicalis F1
( Nakayama et al., 2017 )
agrees with MO data
agrees with MO data, ENU
xnc10
2019 laevis
( Banach et al., 2019 )
or natural mutation
cell line knockout
p2ry4
2017 laevis
F0
( Harata et al., 2019 )
dsp
2019 laevis
F0
( Bharathan and Dickinson,
mad1
2017 tropicalis F2
( Okada et al., 2017 )
2019 )
tyr
2017 laevis
F1
( Ratzan et al., 2017 )
agrees with MO data
L and S mutants from oocyte
l1cam, crb2
2019 tropicalis F0
( Date et al., 2019 )
host transfer. Compound
dnmbp
2019 laevis
F0
( DeLay et al., 2019 )
hets
MO as well
thra
2017 tropicalis F2
( Wen et al., 2017 )
tbx4
2019 tropicalis F0
( Kariminejad et al., 2019 )
tbxt/tbxt.2
2018 tropicalis F1/F4 ( Gentsch et al., 2018 )
link to clinic, limb defect
F1 mutants for tbxt; tbxt/tbxt2
neurod2
2019 tropicalis F0
( Sega et al., 2019 )
double knockouts in F4
link to clinic; RNA
thra/thrb
2018 tropicalis F2/F1 ( Nakajima et al., 2018 )
overexpression
mecom
2018 tropicalis F2
( Okada and Shi, 2018 )
six1
2019 tropicalis F0
( Sullivan et al., 2019 )
pomc
2020 tropicalis F1
( Shewade et al., 2020 )
rpe65, gnat1
2019 laevis
F0
( Wen et al., 2019 )
compound het
ctnnd1
2020 tropicalis F0
( Alharatani et al., 2020 )
prmt1
2020 tropicalis F2
( Shibata et al., 2019 )
trio
2020 tropicalis F0
( Barbosa et al., 2020 )
thra
2021 tropicalis F2
( Tanizaki et al., 2021 b)
link to clinic, domain specif c
CRISPR
effect
tyr
tyr
cela1.2, ets1, ets2,
hspa5, hhex, pgat,
pdx1, ptf1a,
2013
2013
2014
tropicalis F0/F1 ( Blitz et al., 2013 )
tropicalis F0/F1 ( Nakayama et al., 2013 )
tropicalis F0/F1 ( Guo et al., 2014 )
pax9
tnnc1
dlg5
rb1, rbl1, tp53
2020
2020
2020
2020
tropicalis
tropicalis
tropicalis
tropicalis
F0
F0
F0
F0
( Farley-Barnes et al., 2020 )
( Landim-Vieira et al., 2020 )
(Marquez et al., 2021 )
link to clinic
( Naert et al., 2020a )
tm4sf4, tyr
foxj1, pax8, dnah9,
galnt1, ctnnb1
tubb2b, tyr
ptf1a, tyr
gsc, tyr
cfap299
pax3, snai1,
ctnnb1, sox9,
tfap2a, pax3, zic1,
snai2,
rb1, rbl1
2015
2015
2015
2016
2016
2016
2016
tropicalis F0
( Bhattacharya et al., 2015 )
agrees with MO data
tropicalis F0/F1 ( Shi et al., 2015 )
knockin GFP, ela-GFP
laevis
F0
( Wang et al., 2015 )
agrees with MO data
tropicalis F0/F1 ( Blitz et al., 2016 )
leapfrogging, F0 and F1
phenotypes
laevis
F0
( Jaffe et al., 2016 )
tropicalis F0
( Liu et al., 2016 )
tropicalis F0
( Naert et al., 2016 )
dual knockout
multiple
junB
cfap43, foxj1
daam2
thrb
thra/thrb
adprhl1
ednra, ednrb2,
edn1, edn3
nr3c1
2020
2020
2020
2020
2020
2020
2020
2020
2020
cancer
both
( Naert et al., 2020b )
designing eff cient guides
tropicalis F0/F1 ( Nakamura et al., 2020 )
compound hets
laevis
F0
( Rachev et al., 2020 )
agrees with MO data
tropicalis F0
( Schneider et al., 2020 )
link to clinic
tropicalis F2
( Shibata et al., 2020a )
tropicalis F2
( Shibata et al., 2020b )
double knockout
laevis
F0
( Smith et al., 2020 )
agrees with MO data
laevis
F0
( Square et al., 2020 )
tropicalis F2
( Sterner et al., 2020 )
ctnnb1
2017 both
F0/F1 ( Aslan et al., 2017 )
knock in by oocyte host
transfer
ncoa3
gja8, dnase2b
2020
2020
tropicalis
laevis
F1
F0
( Tanizaki et al., 202 1a)
compound hets
( Viet et al., 2020 )
xnc genes
rho
2017
2017
laevis
laevis
F0
( Banach et al., 2017 )
F0/F1 ( Feehan et al., 2017 )
Msmb.3
mtnr1a
2020
2020
tropicalis F0
( Wang et al., 2020 )
agrees with MO data
tropicalis F0/F1 ( Wiechmann et al., 2020 )
(Continued)
(Continued)