115
hey1
hes7.3/esr5
hes7.2
Anterior part of prospective somites S-II (somitomere) and S-III (TZ). Broad domain
in TBD ( Jen et al., 1999 ).
hes7.2 regulation:
dlc overexpression, RBPJ-Ank, or NICD1: expanded the hes7.2 domains into the gaps between
stripes in the somitomeric region ( Jen et al., 1999 ; Peres et al., 2006 ).
RBPJ DBM: ↓hes7.2 throughout the PSM ( Jen et al., 1999 ; Peres
et al., 2006 ).
CHX treatment : continuous hes7.2 expression in the PSM ( Jen et al., 1999 ).
Anterior part of prospective somites S-II (somitomere) and S-III (TZ). Broad domain
in TBD. Segmented prepattern already detected at late gastrula (NF12) ( Sparrow et al.,
1998 ; Jen et al., 1999 )
Gain-of-function:
hes7.3/esr5 overexpression : ↓dlc and hes7.2 in the somitomeric region but activated dlc more
anteriorly, with a net result of a rostral shift of the dlc domain ( Jen et al., 1999 ).
Loss-of-function:
DN-hes7.3/esr5: impaired segmentation and expanded dlc, hes7.2, and mespa expression into the gaps
between stripes in the TZ ( Jen et al., 1999 ).
hes7.3/esr5 regulation:
dlc overexpression, RBPJ-Ank or NICD1: expanded hes7.3/esr5 domains into the gaps between
stripes in the somitomeric region (Jen et al., 1999; Peres et al., 2006).
RBPJ DBM: ↓hes7.3/esr5 in the somitomeric and TZ regions (Jen
et al., 1999; Peres et al., 2006).
CHX treatment : continuous hes7.2 expression in the PSM ( Jen et al., 1999 ).
mespa overexpression : segmental hes7.3/esr5 pattern lost. hes7.3/esr5 in TBD unaffected
(Sparrow et al., 1998).
RA or SU5402 (inhibitor of FGF signaling) treatment: caudal shift of the hes7.3/esr5 TBD
domain (Moreno and Kintner, 2004).
DN-RARα: rostral shift of the hes7.3/esr5 TBD domain (Moreno and Kintner, 2004).
RARβ2 MO: ↓somite number, ↑somite size, and rostrally expanded PSM markers, including
hes7.3/esr5 (Janesick et al., 2017).
ripply2.1 overexpression : slight posterior shift of hes7.3/esr5 expression in the PSM (Chan et al.,
2006 ).
ripply2.1 MO: anterior shift of hes7.3/esr5 expression in the PSM (Chan et al., 2006).
Mature somites (apparently in the caudal half), from NF17 ( Rones et al., 2002 ; Pichon
et al., 2002 ).
Notch Signaling in Early Embryogenesis
hey1
hes7.3/esr5
hes7.2
Anterior part of prospective somites S-II (somitomere) and S-III (TZ). Broad domain
in TBD ( Jen et al., 1999 ).
hes7.2 regulation:
dlc overexpression, RBPJ-Ank, or NICD1: expanded the hes7.2 domains into the gaps between
stripes in the somitomeric region ( Jen et al., 1999 ; Peres et al., 2006 ).
RBPJ DBM: ↓hes7.2 throughout the PSM ( Jen et al., 1999 ; Peres
et al., 2006 ).
CHX treatment : continuous hes7.2 expression in the PSM ( Jen et al., 1999 ).
Anterior part of prospective somites S-II (somitomere) and S-III (TZ). Broad domain
in TBD. Segmented prepattern already detected at late gastrula (NF12) ( Sparrow et al.,
1998 ; Jen et al., 1999 )
Gain-of-function:
hes7.3/esr5 overexpression : ↓dlc and hes7.2 in the somitomeric region but activated dlc more
anteriorly, with a net result of a rostral shift of the dlc domain ( Jen et al., 1999 ).
Loss-of-function:
DN-hes7.3/esr5: impaired segmentation and expanded dlc, hes7.2, and mespa expression into the gaps
between stripes in the TZ ( Jen et al., 1999 ).
hes7.3/esr5 regulation:
dlc overexpression, RBPJ-Ank or NICD1: expanded hes7.3/esr5 domains into the gaps between
stripes in the somitomeric region (Jen et al., 1999; Peres et al., 2006).
RBPJ DBM: ↓hes7.3/esr5 in the somitomeric and TZ regions (Jen
et al., 1999; Peres et al., 2006).
CHX treatment : continuous hes7.2 expression in the PSM ( Jen et al., 1999 ).
mespa overexpression : segmental hes7.3/esr5 pattern lost. hes7.3/esr5 in TBD unaffected
(Sparrow et al., 1998).
RA or SU5402 (inhibitor of FGF signaling) treatment: caudal shift of the hes7.3/esr5 TBD
domain (Moreno and Kintner, 2004).
DN-RARα: rostral shift of the hes7.3/esr5 TBD domain (Moreno and Kintner, 2004).
RARβ2 MO: ↓somite number, ↑somite size, and rostrally expanded PSM markers, including
hes7.3/esr5 (Janesick et al., 2017).
ripply2.1 overexpression : slight posterior shift of hes7.3/esr5 expression in the PSM (Chan et al.,
2006 ).
ripply2.1 MO: anterior shift of hes7.3/esr5 expression in the PSM (Chan et al., 2006).
Mature somites (apparently in the caudal half), from NF17 ( Rones et al., 2002 ; Pichon
et al., 2002 ).
Notch Signaling in Early Embryogenesis