TABLE 7.12
Notch Pathway Genes Expressed during Xenopus Somitogenesis
See abbreviations in Table 7.3 legend.
Expression Related to Somitogenesis and Mature Somites
Functional Evidence Related to Somitogenesis
dll1
RBPJ
notch1
Gene
Continuous in PSM and TBD. One-half of mature somites ( Chitnis et al., 1995 ;
McLaughlin et al., 2000 ; Rones et al., 2002 ; Xenbase, community submitted images:
www.xenbase.org/ , RRID:SCR_003280; Bowes et al., 2010 ).
Gain-of-function:
NICD1 : expanded hes7.2 and hes7.3/esr5 domains into the gaps between stripes in the
somitomeric region ( Jen et al., 1999 ; Peres et al., 2006 ); expanded rnd1, ↓rnd3 ( Goda et al.,
2009 ).
notch1-ΔE: ↓ripply2.2 in the PSM ( Kondow et al., 2006 ).
GR-NICD1; Dex NF11 or 19: ↑hey1 in somites, global disorganization of somite borders ( Rones
et al., 2002 ).
Gain-of-function:
RBPJ-Ank : ↓dlc along the PSM ( Sparrow et al., 1998 ); expanded the hes7.2 and hes7.3/esr5
domains into the gaps between stripes in the somitomeric region ( Jen et al., 1999 ; Peres et al.,
2006 ).
GR-RBPJ-VP16 (Dex NF11–13, Dex NF15–19, ISH NF24–30): ↑hey1 in somites, global
disorganization of somite borders ( Rones et al., 2002 ).
Loss-of-function:
RBPJ DBM: expanded dlc domains into the gaps between somitomeric stripes without affecting
dlc in the TBD ( Jen et al., 1997 ; Sparrow et al., 1998 ); ↓hes4 in somitomeres ( Jen et al.,
1997 ); ↓hes7.2 along the PSM ( Jen et al., 1999 ; Peres et al., 2006 ); ↓hes7.3/esr5 in the
somitomeric and TZ regions ( Jen et al., 1999 ; Peres et al., 2006 ); produced a fusion of both
ripply2.2 somitomeric stripes ( Kondow et al., 2006 ); ↓ripply2.1 in the PSM ( Chan et al.,
2006 ); disrupted rnd3 expression ( Goda et al., 2009 ).
GR-RBPJ DBM (Dex NF11–13, Dex NF15–19, ISH NF24–30): ↓hey1 in somites ( Rones et al.,
2002 ).
RBPJ regulation:
Celf1 controls somitogenesis through RBPJ mRNA decay (see main text for details) ( GautierCourteille et al., 2004 ; Cibois et al., 2010 ; Cibois et al., 2013 ).
More restricted than dlc. Circumblastoporal collar/TBD. Two somitomeric stripes
dll1 regulation:
(from NF12): stronger in the posterior than in the anterior one ( Chitnis et al., 1995 ;
ripply2.1 overexpression : slight posterior shift of dll1 in the PSM ( Chan et al., 2006 ).
McLaughlin et al., 2000 ; Rones et al., 2002 ; Lamar and Kintner, 2005 ; Dingwell and
ripply2.1 MO : anterior shift of dll1 in the PSM ( Chan et al., 2006 ).
Smith, 2006 ; Kondow et al., 2007 ; Wang et al., 2007 ).
ripply2.2 MO : ↓dll1 stripes in the PSM ( Kondow et al., 2007 ).
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Xenopus
Notch Pathway Genes Expressed during Xenopus Somitogenesis
See abbreviations in Table 7.3 legend.
Expression Related to Somitogenesis and Mature Somites
Functional Evidence Related to Somitogenesis
dll1
RBPJ
notch1
Gene
Continuous in PSM and TBD. One-half of mature somites ( Chitnis et al., 1995 ;
McLaughlin et al., 2000 ; Rones et al., 2002 ; Xenbase, community submitted images:
www.xenbase.org/ , RRID:SCR_003280; Bowes et al., 2010 ).
Gain-of-function:
NICD1 : expanded hes7.2 and hes7.3/esr5 domains into the gaps between stripes in the
somitomeric region ( Jen et al., 1999 ; Peres et al., 2006 ); expanded rnd1, ↓rnd3 ( Goda et al.,
2009 ).
notch1-ΔE: ↓ripply2.2 in the PSM ( Kondow et al., 2006 ).
GR-NICD1; Dex NF11 or 19: ↑hey1 in somites, global disorganization of somite borders ( Rones
et al., 2002 ).
Gain-of-function:
RBPJ-Ank : ↓dlc along the PSM ( Sparrow et al., 1998 ); expanded the hes7.2 and hes7.3/esr5
domains into the gaps between stripes in the somitomeric region ( Jen et al., 1999 ; Peres et al.,
2006 ).
GR-RBPJ-VP16 (Dex NF11–13, Dex NF15–19, ISH NF24–30): ↑hey1 in somites, global
disorganization of somite borders ( Rones et al., 2002 ).
Loss-of-function:
RBPJ DBM: expanded dlc domains into the gaps between somitomeric stripes without affecting
dlc in the TBD ( Jen et al., 1997 ; Sparrow et al., 1998 ); ↓hes4 in somitomeres ( Jen et al.,
1997 ); ↓hes7.2 along the PSM ( Jen et al., 1999 ; Peres et al., 2006 ); ↓hes7.3/esr5 in the
somitomeric and TZ regions ( Jen et al., 1999 ; Peres et al., 2006 ); produced a fusion of both
ripply2.2 somitomeric stripes ( Kondow et al., 2006 ); ↓ripply2.1 in the PSM ( Chan et al.,
2006 ); disrupted rnd3 expression ( Goda et al., 2009 ).
GR-RBPJ DBM (Dex NF11–13, Dex NF15–19, ISH NF24–30): ↓hey1 in somites ( Rones et al.,
2002 ).
RBPJ regulation:
Celf1 controls somitogenesis through RBPJ mRNA decay (see main text for details) ( GautierCourteille et al., 2004 ; Cibois et al., 2010 ; Cibois et al., 2013 ).
More restricted than dlc. Circumblastoporal collar/TBD. Two somitomeric stripes
dll1 regulation:
(from NF12): stronger in the posterior than in the anterior one ( Chitnis et al., 1995 ;
ripply2.1 overexpression : slight posterior shift of dll1 in the PSM ( Chan et al., 2006 ).
McLaughlin et al., 2000 ; Rones et al., 2002 ; Lamar and Kintner, 2005 ; Dingwell and
ripply2.1 MO : anterior shift of dll1 in the PSM ( Chan et al., 2006 ).
Smith, 2006 ; Kondow et al., 2007 ; Wang et al., 2007 ).
ripply2.2 MO : ↓dll1 stripes in the PSM ( Kondow et al., 2007 ).
112
Xenopus