97
hey1
hes7.3/
hes6.1
hes5.6
esr5
Germ layers:
Possible regulation of the timing of cardiac f eld specif cation
( Miazga and McLaughlin, 2009 ).
DML:
Possible role in DML development.
Germ layers:
GR-hes5.6-VP16 (Dex NF10): ↓gata4 (mid-gastrula) ( Miazga
and McLaughlin, 2009 ).
DML:
hes5.6, dorsal overexpression : ↑chrd (GO) ( Taelman et al.,
2004 ).
Required for presumptive paraxial mesoderm specif cation,
involving recruitment of TLE/Groucho co-repressors but
not DNA binding.
hes6.1 overexpression : ↑tbxt (pan-mesoderm), myod1
(presumptive paraxial mesoderm), and wnt8a (lateral/
ventral mesoderm) but did not affect the GO marker chrd.
Expansion was restricted to the nearby MZ (ISH, gastrula).
↑tbxt and wnt8a but not chrd or endodermal markers
(animal caps, RT-qPCR). Mesodermal induction by hes6.1
mainly required FGF but also Nodal signaling (RT-qPCR,
ISH) ( Murai et al., 2007 ).
hes6.1 DBM : same effects on mesoderm as hes6.1
overexpression (gastrula).
hes6.1 ΔWRPW: mesodermal markers unaffected (gastrula).
hes6.1 MO: ↓myod1, myf5 (presumptive paraxial mesoderm)
in the IMZ (without affecting tbxt, wnt8a, chrd, or the
endodermal marker sox17). This effect was rescued by
hes6.1 or hes6.1 DBM but not by hes6.1 ΔWRPW (ISH,
mid-gastrula) ( Murai et al., 2007 ).
Necessary for mesoderm specif cation.
hes7.3/esr5 overexpression : ↑tbxt in the IMZ at early gastrula
hes7.3/esr5 ΔWRPW: ↓tbxt at early gastrula (NF10.5, ISH)
(NF10.5, ISH) ( Kinoshita et al., 2011 ).
( Kinoshita et al., 2011 ).
Possible role in the segregation of DML precursors,
repressing notochordal fates.
Hey1 does not bind the co-repressor TLE/Groucho, as it lacks
the typical WRPW motif of bHLH-O repressors ( Pichon et
al., 2004 ). For chrd downregulation, Hey1 acts as a DNA
binding repressor, requiring the Orange domain and the
C-terminal region for dimerization. Hey1 heterodimerizes
in vivo with Hes1 and Hes4 and weakly with Hes2, but it
does not bind Hes5.5 or Hes5.6 ( Taelman et al., 2004 ).
hey1 overexpression : stopped gastrulation, ↓chrd (GO), tbxt
(pan-mesoderm; IMZ), and not in the notochord and GRP.
It did not affect not in the notoplate but induced ectopic not
in the limit of involution, outside the GO ( Taelman et al.,
2004 ).
GR-hey1 (Dex NF12): FP not affected at tailbud stage
( Taelman et al., 2004 ). An earlier induction with Dex will
be useful to address possible effects during DML
segregation.
hey1 DBM : did not repress chrd ( Taelman et al., 2004 ).
Notch Signaling in Early Embryogenesis
hey1
hes7.3/
hes6.1
hes5.6
esr5
Germ layers:
Possible regulation of the timing of cardiac f eld specif cation
( Miazga and McLaughlin, 2009 ).
DML:
Possible role in DML development.
Germ layers:
GR-hes5.6-VP16 (Dex NF10): ↓gata4 (mid-gastrula) ( Miazga
and McLaughlin, 2009 ).
DML:
hes5.6, dorsal overexpression : ↑chrd (GO) ( Taelman et al.,
2004 ).
Required for presumptive paraxial mesoderm specif cation,
involving recruitment of TLE/Groucho co-repressors but
not DNA binding.
hes6.1 overexpression : ↑tbxt (pan-mesoderm), myod1
(presumptive paraxial mesoderm), and wnt8a (lateral/
ventral mesoderm) but did not affect the GO marker chrd.
Expansion was restricted to the nearby MZ (ISH, gastrula).
↑tbxt and wnt8a but not chrd or endodermal markers
(animal caps, RT-qPCR). Mesodermal induction by hes6.1
mainly required FGF but also Nodal signaling (RT-qPCR,
ISH) ( Murai et al., 2007 ).
hes6.1 DBM : same effects on mesoderm as hes6.1
overexpression (gastrula).
hes6.1 ΔWRPW: mesodermal markers unaffected (gastrula).
hes6.1 MO: ↓myod1, myf5 (presumptive paraxial mesoderm)
in the IMZ (without affecting tbxt, wnt8a, chrd, or the
endodermal marker sox17). This effect was rescued by
hes6.1 or hes6.1 DBM but not by hes6.1 ΔWRPW (ISH,
mid-gastrula) ( Murai et al., 2007 ).
Necessary for mesoderm specif cation.
hes7.3/esr5 overexpression : ↑tbxt in the IMZ at early gastrula
hes7.3/esr5 ΔWRPW: ↓tbxt at early gastrula (NF10.5, ISH)
(NF10.5, ISH) ( Kinoshita et al., 2011 ).
( Kinoshita et al., 2011 ).
Possible role in the segregation of DML precursors,
repressing notochordal fates.
Hey1 does not bind the co-repressor TLE/Groucho, as it lacks
the typical WRPW motif of bHLH-O repressors ( Pichon et
al., 2004 ). For chrd downregulation, Hey1 acts as a DNA
binding repressor, requiring the Orange domain and the
C-terminal region for dimerization. Hey1 heterodimerizes
in vivo with Hes1 and Hes4 and weakly with Hes2, but it
does not bind Hes5.5 or Hes5.6 ( Taelman et al., 2004 ).
hey1 overexpression : stopped gastrulation, ↓chrd (GO), tbxt
(pan-mesoderm; IMZ), and not in the notochord and GRP.
It did not affect not in the notoplate but induced ectopic not
in the limit of involution, outside the GO ( Taelman et al.,
2004 ).
GR-hey1 (Dex NF12): FP not affected at tailbud stage
( Taelman et al., 2004 ). An earlier induction with Dex will
be useful to address possible effects during DML
segregation.
hey1 DBM : did not repress chrd ( Taelman et al., 2004 ).
Notch Signaling in Early Embryogenesis