89
hes7.3/esr5
hes7.2
hes7.1
hes5.7
hes5.4
hes5.2
hes7.1 MO: f lled the MHB gap with hes5.1
expression (PN medial stripes) and anteriorly
expanded the hes5.1 PN intermediate stripes
( Takada et al., 2005 ).
hes7.3/esr5
Negative during mesoderm induction.
hes7.3/esr5-ΔWRPW : ↑hes5.1 in animal caps
mesodermalized by nodal2 (NF10.5, sqRT-PCR)
and in the MZ (NF10.5, ISH) ( Kinoshita et al.,
2011 ).
hes6.1
Positive during PN.
hes6.1 overexpression : ↑hes5.2 PN domains [data not shown
in ( Koyano-Nakagawa et al., 2000 )].
hes7.1
Represses hes5.2 (probably directly) during the
hes7.1 overexpression : ↓hes5.2 (neural plate stage)
GR-hes7.1-VP16 (Dex NF10.5–11) +/− CHX :
establishment of the presumptive MHB.
( Takada et al., 2005 ).
↑hes5.2 domains at neural plate stage ( Takªda et
al., 2005 ).
hes5.4
Auto-repression
hes5.4 MO: ectopic hes5.4 in the neural plate
( Riddiford and Schlosser, 2017 ).
hes7.1
Represses hes5.7 (probably directly) during the
hes7.1 overexpression : ↓hes5.7 (neural plate stage)
GR-hes7.1-VP16 (Dex NF10.5–11) +/− CHX :
establishment of the presumptive MHB.
( Takada et al., 2005 ).
↑hes5.7 domains at neural plate stage ( Takada et
al., 2005 ).
hes7.1 MO : f lled the MHB gap with hes5.7
expression (medial stripes of PN) and anteriorly
expanded the hes5.7 PN intermediate stripes
( Takada et al., 2005 ).
hes5.1
Negative during MHB specif cation
hes5.1 overexpression : ↓hes7.1 (MHB, ISH at neural
plate stage ( Takada et al., 2005 )
hes7.1
Auto-repression (probably direct) during the establishment
GR-hes7.1-VP16 (Dex NF10.5–11) +/− CHX:
of the presumptive MHB.
↑hes7.1 domains at neural plate stage ( Takada et
al., 2005 ).
hes7.3/esr5
Negative in somitogenesis.
hes7.3/esr5 overexpression: ↓hes7.2 in the PSM ( Jen et hes7.3/esr5-ΔWRPW: derepressed hes7.2 in the TZ
Negative feedback loop of Notch pathway
al., 1999 )
in the PSM ( Jen et al., 1999 ).
notch1/
Negative during mesoderm induction
NICD1: unable to induce hes7.3/esr5 in naive animal
hes5.1-ΔWRPW : ↑hes7.3/esr5 (animal caps
hes5.1
caps.
mesodermalized by nodal2, NF10.5, sqRT-PCR)
NICD1 or hes5.1 overexpression: ↓hes7.3/esr5 induced
( Kinoshita et al., 2011 ).
by the mesodermal inducer nodal2 (animal caps,
NF10.5, sqRT-PCR).
hes5.1 overexpression: ↓hes7.3/esr5 in the IMZ
(NF10.5, ISH)
( Kinoshita et al., 2011 ).
Notch Signaling in Early Embryogenesis
hes7.3/esr5
hes7.2
hes7.1
hes5.7
hes5.4
hes5.2
hes7.1 MO: f lled the MHB gap with hes5.1
expression (PN medial stripes) and anteriorly
expanded the hes5.1 PN intermediate stripes
( Takada et al., 2005 ).
hes7.3/esr5
Negative during mesoderm induction.
hes7.3/esr5-ΔWRPW : ↑hes5.1 in animal caps
mesodermalized by nodal2 (NF10.5, sqRT-PCR)
and in the MZ (NF10.5, ISH) ( Kinoshita et al.,
2011 ).
hes6.1
Positive during PN.
hes6.1 overexpression : ↑hes5.2 PN domains [data not shown
in ( Koyano-Nakagawa et al., 2000 )].
hes7.1
Represses hes5.2 (probably directly) during the
hes7.1 overexpression : ↓hes5.2 (neural plate stage)
GR-hes7.1-VP16 (Dex NF10.5–11) +/− CHX :
establishment of the presumptive MHB.
( Takada et al., 2005 ).
↑hes5.2 domains at neural plate stage ( Takªda et
al., 2005 ).
hes5.4
Auto-repression
hes5.4 MO: ectopic hes5.4 in the neural plate
( Riddiford and Schlosser, 2017 ).
hes7.1
Represses hes5.7 (probably directly) during the
hes7.1 overexpression : ↓hes5.7 (neural plate stage)
GR-hes7.1-VP16 (Dex NF10.5–11) +/− CHX :
establishment of the presumptive MHB.
( Takada et al., 2005 ).
↑hes5.7 domains at neural plate stage ( Takada et
al., 2005 ).
hes7.1 MO : f lled the MHB gap with hes5.7
expression (medial stripes of PN) and anteriorly
expanded the hes5.7 PN intermediate stripes
( Takada et al., 2005 ).
hes5.1
Negative during MHB specif cation
hes5.1 overexpression : ↓hes7.1 (MHB, ISH at neural
plate stage ( Takada et al., 2005 )
hes7.1
Auto-repression (probably direct) during the establishment
GR-hes7.1-VP16 (Dex NF10.5–11) +/− CHX:
of the presumptive MHB.
↑hes7.1 domains at neural plate stage ( Takada et
al., 2005 ).
hes7.3/esr5
Negative in somitogenesis.
hes7.3/esr5 overexpression: ↓hes7.2 in the PSM ( Jen et hes7.3/esr5-ΔWRPW: derepressed hes7.2 in the TZ
Negative feedback loop of Notch pathway
al., 1999 )
in the PSM ( Jen et al., 1999 ).
notch1/
Negative during mesoderm induction
NICD1: unable to induce hes7.3/esr5 in naive animal
hes5.1-ΔWRPW : ↑hes7.3/esr5 (animal caps
hes5.1
caps.
mesodermalized by nodal2, NF10.5, sqRT-PCR)
NICD1 or hes5.1 overexpression: ↓hes7.3/esr5 induced
( Kinoshita et al., 2011 ).
by the mesodermal inducer nodal2 (animal caps,
NF10.5, sqRT-PCR).
hes5.1 overexpression: ↓hes7.3/esr5 in the IMZ
(NF10.5, ISH)
( Kinoshita et al., 2011 ).
Notch Signaling in Early Embryogenesis