Analytica Chimica Acta, accepted, 07/07/2015. This is the accepted version without proofing
corrections. DOI: 10.1016/j.aca.2015.06.011
.
Page 19 of 26
Figure S-7: TSFS spectra of HSA (1 mg mL
–1 ) in PBS at 20ºC: a) HH, b) VV, c) VH, d) HV polarized, and e)
Standard (non-polarized)TSFS spectrum.
Note: Polarizer filters & effect on excitation spectra.
The polarizers used in the Cary Eclipse are polymer based thin film based and have a spectral
cut-off at ~290 nm (see Figure S-3). Below this most of (>99%) the excitation light is
absorbed by the filter material and thus there is no excitation. Likewise, in the emission
pathway, filter absorption starts to become significant below ~300 nm.
corrections. DOI: 10.1016/j.aca.2015.06.011
.
Page 19 of 26
Figure S-7: TSFS spectra of HSA (1 mg mL
–1 ) in PBS at 20ºC: a) HH, b) VV, c) VH, d) HV polarized, and e)
Standard (non-polarized)TSFS spectrum.
Note: Polarizer filters & effect on excitation spectra.
The polarizers used in the Cary Eclipse are polymer based thin film based and have a spectral
cut-off at ~290 nm (see Figure S-3). Below this most of (>99%) the excitation light is
absorbed by the filter material and thus there is no excitation. Likewise, in the emission
pathway, filter absorption starts to become significant below ~300 nm.