Multiple coverslips submerged in swirling liquid cultures were uniformly colonized by
encapsulated cells of a sediment isolate of E. cloacae. The colonized coverslips provided a
means of determining glucose uptake characteristics of the adherent cells shortly after
their attachment. The technique should also be useful in evaluating other physiological
responses elicited by bacteria when they switch from a free-living to a sessile existence.
BRIGHT J.J. and M. FLETCHER, 1983. Amino acid assimilation and respiration by attached and free-living
populations of a marine Pseudomonas sp. Microb. ecol. 9 : 215-226.
COSTERTON J. W., R.T. IRVIN, and K.-J.CHENG, 1981. The bacterial glycocalyx in nature and disease. Ann. Rev.
Microbiol. 35 : 299-324.
GEESEY G.G., R. MUTCH, J.W. COSTERTON, R.B. GREEN, 1978. Sessile bacteria : an important component of the
microbial population in small mountain steams. Limnol. Oceanogr. 23:1214-1223.
MORIARTY D.J.W. and A.C. HAYWARD, 1982. Ultrastructure of bacteria and the proportion of Gram-negative
bacteria in marine sediments. Microb. Ecol. 8 .1-14.
SALAS S.D.and G.G.GEESEY, 1983. Surface attachment of a sediment isolate of Enterobacter cloacae. Microb.
Ecol. 9 : 307-315.
ZOBELL C.E, 1943. The effect of solid surfaces upon bacterial activity. J. Bacterial. 46 :39-56.
247
encapsulated cells of a sediment isolate of E. cloacae. The colonized coverslips provided a
means of determining glucose uptake characteristics of the adherent cells shortly after
their attachment. The technique should also be useful in evaluating other physiological
responses elicited by bacteria when they switch from a free-living to a sessile existence.
BRIGHT J.J. and M. FLETCHER, 1983. Amino acid assimilation and respiration by attached and free-living
populations of a marine Pseudomonas sp. Microb. ecol. 9 : 215-226.
COSTERTON J. W., R.T. IRVIN, and K.-J.CHENG, 1981. The bacterial glycocalyx in nature and disease. Ann. Rev.
Microbiol. 35 : 299-324.
GEESEY G.G., R. MUTCH, J.W. COSTERTON, R.B. GREEN, 1978. Sessile bacteria : an important component of the
microbial population in small mountain steams. Limnol. Oceanogr. 23:1214-1223.
MORIARTY D.J.W. and A.C. HAYWARD, 1982. Ultrastructure of bacteria and the proportion of Gram-negative
bacteria in marine sediments. Microb. Ecol. 8 .1-14.
SALAS S.D.and G.G.GEESEY, 1983. Surface attachment of a sediment isolate of Enterobacter cloacae. Microb.
Ecol. 9 : 307-315.
ZOBELL C.E, 1943. The effect of solid surfaces upon bacterial activity. J. Bacterial. 46 :39-56.
247