Biomarkers
Lipid content and fatty acid composition
of two cones from the Indian Ocean: Conus
barthelemy and C. striatus
Gilles Barnathan (1) , Céline Orhon
(l) , Maurice Aknin
(2)
(1) Faculté de pharmacie, groupe SMAB, ISOMer, laboratoire de chimie marine,
1 rue Gaston Veil, BP 1024, 44035 Nantes Cedex 01, France
(2) Centre universitaire, IUFM, 97487 Saint-Denis, La Réunion, France
Abstract
Lipid content and fatty acid composition were similar in two Indian
Ocean cones, C onus barthelemy and C. striatus. Phospholipids, triglycerides and free fatty acids contained major amounts of saturated straightchain acids (67-72% of the fatty acid mixtures). Forty fatty acids
ranging from C12 ro C25 were idenrified. The usual marine polyunsaturated fatty acids occurred in very low amounts or not at all, except
arachidonic acid which was present in phospholipids at significant
levels (1.8-3.2%). Unusual acids were found, such as the Δ5,l 1-20:2,
Δ7,13-22:2 and Δ7,15-22:2 acids and several Δ7 monounsaturated acids.
Résumé
La composition en lipides et en acides gras a été étudiée chez deux cônes
de l'océan Indien, Conus barthelemy et C. striatus, et a été trouvée similaire. Phospholipides, triglycérides et acides gras libres contenaient une
majeure parrie d’acides saturés à chaîne normale (67-72 % des mélanges
d’acides gras). Quarante acides gras, de C12 à C25, ont été identifiés
par couplage CG/SM à l’état d’esters méthyliques et de N-acyl pyrrolidides. Les acides gras polyinsaturés marins habituels n’étaient présents
qu’en petites quantités ou absents, excepté l’acide arachidonique présent dans les phospholipides en pourcentages notables (1,8-3,2%). Des
acides gras inhabituels ont été trouvés tels que les acides Δ5,11-20:2,
Δ7,13-22:2, Δ7,15-22:2 et plusieurs acides monoinsaturés Δ7.
Introduction
The family of Conidae comprises a great number of predacious marine
snails which possess a specialized venom apparatus to immobilize their
prey. Though many reports have been published on the peptide conotoxins of cones (Myers et al., 1993), little is known about the chemical
nature of rhe subsrance responsible for rhe contractile effect. Despite
the fact that a large amount of arachidonic acid has been found in rhe
venom duct of C. textile as an active substance, the principle of the
63
Lipid content and fatty acid composition
of two cones from the Indian Ocean: Conus
barthelemy and C. striatus
Gilles Barnathan (1) , Céline Orhon
(l) , Maurice Aknin
(2)
(1) Faculté de pharmacie, groupe SMAB, ISOMer, laboratoire de chimie marine,
1 rue Gaston Veil, BP 1024, 44035 Nantes Cedex 01, France
(2) Centre universitaire, IUFM, 97487 Saint-Denis, La Réunion, France
Abstract
Lipid content and fatty acid composition were similar in two Indian
Ocean cones, C onus barthelemy and C. striatus. Phospholipids, triglycerides and free fatty acids contained major amounts of saturated straightchain acids (67-72% of the fatty acid mixtures). Forty fatty acids
ranging from C12 ro C25 were idenrified. The usual marine polyunsaturated fatty acids occurred in very low amounts or not at all, except
arachidonic acid which was present in phospholipids at significant
levels (1.8-3.2%). Unusual acids were found, such as the Δ5,l 1-20:2,
Δ7,13-22:2 and Δ7,15-22:2 acids and several Δ7 monounsaturated acids.
Résumé
La composition en lipides et en acides gras a été étudiée chez deux cônes
de l'océan Indien, Conus barthelemy et C. striatus, et a été trouvée similaire. Phospholipides, triglycérides et acides gras libres contenaient une
majeure parrie d’acides saturés à chaîne normale (67-72 % des mélanges
d’acides gras). Quarante acides gras, de C12 à C25, ont été identifiés
par couplage CG/SM à l’état d’esters méthyliques et de N-acyl pyrrolidides. Les acides gras polyinsaturés marins habituels n’étaient présents
qu’en petites quantités ou absents, excepté l’acide arachidonique présent dans les phospholipides en pourcentages notables (1,8-3,2%). Des
acides gras inhabituels ont été trouvés tels que les acides Δ5,11-20:2,
Δ7,13-22:2, Δ7,15-22:2 et plusieurs acides monoinsaturés Δ7.
Introduction
The family of Conidae comprises a great number of predacious marine
snails which possess a specialized venom apparatus to immobilize their
prey. Though many reports have been published on the peptide conotoxins of cones (Myers et al., 1993), little is known about the chemical
nature of rhe subsrance responsible for rhe contractile effect. Despite
the fact that a large amount of arachidonic acid has been found in rhe
venom duct of C. textile as an active substance, the principle of the
63