Marine lipids
Potential water soluble vitamin delivery
using liposomes based on marine lipids
M. Cansell
(1) , E. Bailhache
(1) , J.-F. Samain
(2) ,
J. Moal
(2) , J.-P. Gouygou
(1) , B. Entressangles
(1)
(1) Université de Bordeaux 1, ISTAB, laboratoire de lipochimie alimentaire, 1 av. des Facultés,
33405 Talence Cedex, France - m.cansell@istab.u-bordeaux.fr
(2) Ifremer Brest, Drv/ra, laboratoire de physiologie des invertébrés,
BP 70, 29280 Plouzané, France
(3) Ifremer Nantes, Drv/vp, route de l’île d’Yeu, BP 21105, 44311 Nantes Cedex 03, France
Abstract
Liposomes, used as delivery systems of both lipids and hydrosoluble compounds, appear to be potential candidates as artificial diets. For example,
they could supplement lacking essential nutriments in microalgae,
used as a food source in hatchery culture of bivalves. In this context,
vesicles, based on marine lipids and loacled with vitamin B1 (thiamine)
were prepared by extrusion and their stability examined. Liposome
preparation consisted in a mixture of multilamellar and unilamellar
structures with a mean diameter (MD) of 6 micrometres. These morphologic characteristics remained constant over a 2-week period in buffer
solution. However, dilution with sea water induced an immediate formation of large aggregates (MD = 9 pm). Liposome stability was assayed
by membrane permeability to thiamine. Fifty per cent of the vitamin
still remained in the liposome structure after 63 hours of storage in an
aqueous buffer solution while, in presence of sea water, retention was
drastically reduced to three hours. Thiamine retention was significantly
improved by coating liposomes with a dextran grafted with cholesterol
since 150 hours and 18 hours were required to obtained 50% of leakage
in buffer solution and sea water, respectively.
Résumé
En raison de leur structure, les liposomes peuvent être utilisés pour
supplémenter, en composés liposolubles et hydrosolubles, les régimes de
microalgues déficients en nutriments essentiels et servir ainsi de source
nutritive pour lelevage des bivalves en écloserie. Dans cette optique,
cles liposomes à base cle lipides marins et contenant de la vitamine B1
(thiamine) ont été préparés par extrusion. Les liposomes ainsi obtenus
sont un mélange de structures multilamellaires et unilamellaires présentant un diamètre moyen (DM) de 6 micromètres. Les caractéristiques
morphologiques de ces liposomes restent inchangées pendant plus de
deux semaines clans la solution tampon. En revanche, la dilution cles
liposomes dans l’eau de mer conduit à leur agrégation immédiate
212
Potential water soluble vitamin delivery
using liposomes based on marine lipids
M. Cansell
(1) , E. Bailhache
(1) , J.-F. Samain
(2) ,
J. Moal
(2) , J.-P. Gouygou
(1) , B. Entressangles
(1)
(1) Université de Bordeaux 1, ISTAB, laboratoire de lipochimie alimentaire, 1 av. des Facultés,
33405 Talence Cedex, France - m.cansell@istab.u-bordeaux.fr
(2) Ifremer Brest, Drv/ra, laboratoire de physiologie des invertébrés,
BP 70, 29280 Plouzané, France
(3) Ifremer Nantes, Drv/vp, route de l’île d’Yeu, BP 21105, 44311 Nantes Cedex 03, France
Abstract
Liposomes, used as delivery systems of both lipids and hydrosoluble compounds, appear to be potential candidates as artificial diets. For example,
they could supplement lacking essential nutriments in microalgae,
used as a food source in hatchery culture of bivalves. In this context,
vesicles, based on marine lipids and loacled with vitamin B1 (thiamine)
were prepared by extrusion and their stability examined. Liposome
preparation consisted in a mixture of multilamellar and unilamellar
structures with a mean diameter (MD) of 6 micrometres. These morphologic characteristics remained constant over a 2-week period in buffer
solution. However, dilution with sea water induced an immediate formation of large aggregates (MD = 9 pm). Liposome stability was assayed
by membrane permeability to thiamine. Fifty per cent of the vitamin
still remained in the liposome structure after 63 hours of storage in an
aqueous buffer solution while, in presence of sea water, retention was
drastically reduced to three hours. Thiamine retention was significantly
improved by coating liposomes with a dextran grafted with cholesterol
since 150 hours and 18 hours were required to obtained 50% of leakage
in buffer solution and sea water, respectively.
Résumé
En raison de leur structure, les liposomes peuvent être utilisés pour
supplémenter, en composés liposolubles et hydrosolubles, les régimes de
microalgues déficients en nutriments essentiels et servir ainsi de source
nutritive pour lelevage des bivalves en écloserie. Dans cette optique,
cles liposomes à base cle lipides marins et contenant de la vitamine B1
(thiamine) ont été préparés par extrusion. Les liposomes ainsi obtenus
sont un mélange de structures multilamellaires et unilamellaires présentant un diamètre moyen (DM) de 6 micromètres. Les caractéristiques
morphologiques de ces liposomes restent inchangées pendant plus de
deux semaines clans la solution tampon. En revanche, la dilution cles
liposomes dans l’eau de mer conduit à leur agrégation immédiate
212