Biotechnology
Metabolites from marine bacteria
Valérie Bultel-Poncé (1) , Cécile Debitus (2) , Michèle Guyot (1)
(1) Muséum national d’histoire naturelle, laboratoire de chimie des substances naturelles
associé au CNRS, 63 rue Buffon, 75005 Paris, France - bultel@mnhn.fr
(2) IRD Noumea, BP A5, 98848 Nouméa Cedex, New Caledoma
Abstract
In an ongoing survey of bioactive potential of microorganisms associated
with marine invertebrates, the extract of the sponge-associated bacterial
strain Microccocus luteus was found to exhibit potent antimicrobial
activity. The previously known synthetic 2,4,4’-trichloro-2’-hydroxydiphenylether (Zinkernagle & Koenig, 1967) was found to be responsible for the antimicrobial activity. The major metabolite isolated was
a new acyl-l-(acyl-6’-mannobiosyl)-3-glycerol (Bultel-Poncé et al.,
1997, 1998).
Quinolones and a phosphatidylglyceride were isolated from the spongeassociated bacterial strain Pseudomonas sp. Structure elucidation was
performed by spectroscopic analysis and chemical transformations
(Bultel-Poncé et al., 1999).
Résumé
Dans le cadre d’un programme de recherche de composés bio-actifs
extraits de bactéries marines associées à des invertébrés marins, l’étude
de la bactérie Micrococcus luteus, collectée sur une éponge du genre Xestospongia sp., a permis d’identifier le trichloro-2,4,4’-hydroxy-2’-diphényléther (Zinkernagle & Koenig, 1967) responsable de l’activité antibiotique, ainsi que le composé majoritaire de l’extrait, un acyl-l-(mannobiosyl-acyl-6’)-3-glycerol (Bultel-Poncéetal., 1997, 1998). L’étude
d’une nouvelle souche bactérienne du genre Pseudomonas sp. a conduit
à l’identification de c]uatre quinolones et d’un nouveau phosphatidyl
glycéride (Bultel-Poncé et al., 1999).
Introduction
In the last few years marine bacteria emerged as a new field for the discovery of novel biologically active compounds from marine origin
(Fenical, 1993). Isolation of these bacteria can originate mainly from
sediments, but also from open oceans or marine surfaces including
marine living organisms (Jansen & Fenical, 1994). To date only a restricted
number of papers are devoted to the chemical study of bacteria associated
to invertebrates. Bacteria collected from sponges (Stierle et al., 1988;
Stierle & Stierle, 1992; Shigemori et al., 1992; Imamura et al., 1999
193
Metabolites from marine bacteria
Valérie Bultel-Poncé (1) , Cécile Debitus (2) , Michèle Guyot (1)
(1) Muséum national d’histoire naturelle, laboratoire de chimie des substances naturelles
associé au CNRS, 63 rue Buffon, 75005 Paris, France - bultel@mnhn.fr
(2) IRD Noumea, BP A5, 98848 Nouméa Cedex, New Caledoma
Abstract
In an ongoing survey of bioactive potential of microorganisms associated
with marine invertebrates, the extract of the sponge-associated bacterial
strain Microccocus luteus was found to exhibit potent antimicrobial
activity. The previously known synthetic 2,4,4’-trichloro-2’-hydroxydiphenylether (Zinkernagle & Koenig, 1967) was found to be responsible for the antimicrobial activity. The major metabolite isolated was
a new acyl-l-(acyl-6’-mannobiosyl)-3-glycerol (Bultel-Poncé et al.,
1997, 1998).
Quinolones and a phosphatidylglyceride were isolated from the spongeassociated bacterial strain Pseudomonas sp. Structure elucidation was
performed by spectroscopic analysis and chemical transformations
(Bultel-Poncé et al., 1999).
Résumé
Dans le cadre d’un programme de recherche de composés bio-actifs
extraits de bactéries marines associées à des invertébrés marins, l’étude
de la bactérie Micrococcus luteus, collectée sur une éponge du genre Xestospongia sp., a permis d’identifier le trichloro-2,4,4’-hydroxy-2’-diphényléther (Zinkernagle & Koenig, 1967) responsable de l’activité antibiotique, ainsi que le composé majoritaire de l’extrait, un acyl-l-(mannobiosyl-acyl-6’)-3-glycerol (Bultel-Poncéetal., 1997, 1998). L’étude
d’une nouvelle souche bactérienne du genre Pseudomonas sp. a conduit
à l’identification de c]uatre quinolones et d’un nouveau phosphatidyl
glycéride (Bultel-Poncé et al., 1999).
Introduction
In the last few years marine bacteria emerged as a new field for the discovery of novel biologically active compounds from marine origin
(Fenical, 1993). Isolation of these bacteria can originate mainly from
sediments, but also from open oceans or marine surfaces including
marine living organisms (Jansen & Fenical, 1994). To date only a restricted
number of papers are devoted to the chemical study of bacteria associated
to invertebrates. Bacteria collected from sponges (Stierle et al., 1988;
Stierle & Stierle, 1992; Shigemori et al., 1992; Imamura et al., 1999
193