Marine lipids
Table 1 - Effect of sterol addition on the total sterol composition of the spat after 7, 19, 33 days of feeding.
(Mean, SD, n=3). The statistical differences (p<0.05) between treatments are indicated by a letter.
Control
3%
10%
20%
7 days of supplementation Mean SD
Mean SD
Mean SD
Mean SD
Brassicasterol
40.4
2.5
a
40.8
1.1
a
38.7
1.5
a
35. 7
0.5
b
Campesterol
14.5
0.8
14.1
0.4
13.9
0.9
13.8
0.4
24-Methylenecholesterol
6.9
0.4
0.0
0.5
6.3
0.1
0.1
0.4
Stigmasterol
2.6
0.2
a
3.1
0.1
b
4.0
0.2
c
4.8
0.1
d
Cholesterol
21.0
0.5
a
20.7
0.0
a
22.6
0.6
b
24.5
0.9
c
Total sterol µg/individu
27.0
7.2
32.0
8.1
25.0
7.6
26.0 11.5
19 days of supplementation
Brassicasterol
49.1
0.9
48.5
1.6
a
45.4
2.0
41.8
1.1
b
Campesterol
17.2
0.8
16.3
0.6
15.1
1.1
15.2
1.2
24-Methylenecholesterol
5.0
0.1
a
5.2
0.4
a
4. 7
0.1
a
4.5
0.4
b
Stigmasterol
1.0
0.1
a
2.9
0.2
b
4.5
0,0
c
5.5
0.0
d
Cholesterol
15.0
0.5
a
17.1
0.5
b
20.0
0.4
c
22.2
0.2
d
Total sterol µg/individu
69.4 10.3
82.5 11.9
60.7
6.5
67.5
8.2
33 days of supplementation
Brassicasterol
54.0
0.4
a
57.7
3.4
ab
50.2
2.4
ac
48.5
2.7
ac
Campesterol
17.0
0.4
a
13.2
1.5
b
13.4
1.0
b
12.9
1.4
b
24-Methylenecholesterol
5.5
0.3
4.6
0.7
4.0
0.3
4.2
0.5
Stigmasterol
2.2
0.1
a
3.0
0.1
b
4.7
0.2
c
5.8
0.4
d
Cholesterol
11.5
0.4
a
12.5
0.9
a
16.9
0.0
b
10.2
1.7
b
Total sterol pg/individu
90.4
6.8
103.6 32.9
85.6 21.8
88.5 10.3
The total accumulation all over the experiment of each sterol by spat
from algae and emulsion for each treatment was calculated. The dietary
rations of the major algal sterols: brassicasterol, campesterol and 24methylenecholesterol were respectively 54, 26 and 4.5 pg/spat. The
accumulation rates of these sterol algae in all treatments were estimated
from 68% to 90% for the brassicasterol, from 35% to 51% for the
campesterol and from 42% to 75% for the 24-methylenecholesterol.
Total cholesterol and stigmasterol gained by spat during 33 days of
feeding increased as a polynomial function of the total sterol added
with the emulsion per spat (fig.). The accumulation rates of emulsion
sterols ranged from 2.4% to 4.7% for the cholesterol and from 1.1 % to
2.2% for the stigmasterol (table 2). The accumulation of brassicasterol
provided at 54 pg/spat by algae was much higher than the accumulations of cholesterol and stigmasterol provided at a similar range
(50 mg/spat) by emulsion (3% emulsion treatment).
Table 2 - Accumulation percentage of emulsion sterols by spat
after 33 days of experimentation.
3%
10%
20%
Stigmasterol
2.2
1.5
1.1
Cholesterol
4.7
3.0
2.4
126
Table 1 - Effect of sterol addition on the total sterol composition of the spat after 7, 19, 33 days of feeding.
(Mean, SD, n=3). The statistical differences (p<0.05) between treatments are indicated by a letter.
Control
3%
10%
20%
7 days of supplementation Mean SD
Mean SD
Mean SD
Mean SD
Brassicasterol
40.4
2.5
a
40.8
1.1
a
38.7
1.5
a
35. 7
0.5
b
Campesterol
14.5
0.8
14.1
0.4
13.9
0.9
13.8
0.4
24-Methylenecholesterol
6.9
0.4
0.0
0.5
6.3
0.1
0.1
0.4
Stigmasterol
2.6
0.2
a
3.1
0.1
b
4.0
0.2
c
4.8
0.1
d
Cholesterol
21.0
0.5
a
20.7
0.0
a
22.6
0.6
b
24.5
0.9
c
Total sterol µg/individu
27.0
7.2
32.0
8.1
25.0
7.6
26.0 11.5
19 days of supplementation
Brassicasterol
49.1
0.9
48.5
1.6
a
45.4
2.0
41.8
1.1
b
Campesterol
17.2
0.8
16.3
0.6
15.1
1.1
15.2
1.2
24-Methylenecholesterol
5.0
0.1
a
5.2
0.4
a
4. 7
0.1
a
4.5
0.4
b
Stigmasterol
1.0
0.1
a
2.9
0.2
b
4.5
0,0
c
5.5
0.0
d
Cholesterol
15.0
0.5
a
17.1
0.5
b
20.0
0.4
c
22.2
0.2
d
Total sterol µg/individu
69.4 10.3
82.5 11.9
60.7
6.5
67.5
8.2
33 days of supplementation
Brassicasterol
54.0
0.4
a
57.7
3.4
ab
50.2
2.4
ac
48.5
2.7
ac
Campesterol
17.0
0.4
a
13.2
1.5
b
13.4
1.0
b
12.9
1.4
b
24-Methylenecholesterol
5.5
0.3
4.6
0.7
4.0
0.3
4.2
0.5
Stigmasterol
2.2
0.1
a
3.0
0.1
b
4.7
0.2
c
5.8
0.4
d
Cholesterol
11.5
0.4
a
12.5
0.9
a
16.9
0.0
b
10.2
1.7
b
Total sterol pg/individu
90.4
6.8
103.6 32.9
85.6 21.8
88.5 10.3
The total accumulation all over the experiment of each sterol by spat
from algae and emulsion for each treatment was calculated. The dietary
rations of the major algal sterols: brassicasterol, campesterol and 24methylenecholesterol were respectively 54, 26 and 4.5 pg/spat. The
accumulation rates of these sterol algae in all treatments were estimated
from 68% to 90% for the brassicasterol, from 35% to 51% for the
campesterol and from 42% to 75% for the 24-methylenecholesterol.
Total cholesterol and stigmasterol gained by spat during 33 days of
feeding increased as a polynomial function of the total sterol added
with the emulsion per spat (fig.). The accumulation rates of emulsion
sterols ranged from 2.4% to 4.7% for the cholesterol and from 1.1 % to
2.2% for the stigmasterol (table 2). The accumulation of brassicasterol
provided at 54 pg/spat by algae was much higher than the accumulations of cholesterol and stigmasterol provided at a similar range
(50 mg/spat) by emulsion (3% emulsion treatment).
Table 2 - Accumulation percentage of emulsion sterols by spat
after 33 days of experimentation.
3%
10%
20%
Stigmasterol
2.2
1.5
1.1
Cholesterol
4.7
3.0
2.4
126