Biomarkers
Characterization of a new fatty acid
in pectinids
Yanic Marty (1) , Philippe Soudant (2) , Sébastien Perrotte
(1) ,
Jeanne Moal
(2) , Jacques Dussauze
(3) , Jean-François Samain
(2)
(1) Université de Bretagne occidentale, UMR-CNRS 6521, BP 809, 29285 Brest Cedex, France
Yanic.Marty@umv-brest.fr
(2) Ifremer Brest, Drv/ra, laboratoire de physiologie des invertébrés,
BP 70, 29280 Plouzané, France
(3) Pôle analytique des Eaux, technopôle Brest-lroise, 120 av. Alexis de Ronchon,
BP 52, 29280 Plouzané, France
A new fatty acid discovered from the scallop Pecten maximus was isolated
as a methyl ester using silver nitrate liquid chromatography and silicic
high performance liquid chromatography. This fatty acid was determined
to be a 22:4 geometrical isomer [A4?, 7?, 1 O?, 13?} (X) using gas chromatography-mass spectrometry analysis of their 2-alkenyl-4-4-dimethyloxazoline derivatives (GC/MS DMOX). The number of trans- double
bounds of this fatty acid was determined on silver ion high performance liquid chromatography by comparison with the mono-trans
geometrical isomers of 22:4 [A4cis, 7cis, 1 Ocis, 13cis] (S), obtained
through partial hydrazine reduction of all cis-22:6 (n-3) followed by a
p-toluenesulfinic isomerization of the derivatives. Comparing the four
trienes (22:3) obtained through partial hydrazine reduction of the two
compounds (X) and (S) determined that the trans-double bound was
located in Al 3.
This fatty acid identified as cis-4, 7, 10 trans-13-docosatetraenoic acid
has not been reportecl previously in any organisms and is believed to
be specific for the pectinid family.
107
Characterization of a new fatty acid
in pectinids
Yanic Marty (1) , Philippe Soudant (2) , Sébastien Perrotte
(1) ,
Jeanne Moal
(2) , Jacques Dussauze
(3) , Jean-François Samain
(2)
(1) Université de Bretagne occidentale, UMR-CNRS 6521, BP 809, 29285 Brest Cedex, France
Yanic.Marty@umv-brest.fr
(2) Ifremer Brest, Drv/ra, laboratoire de physiologie des invertébrés,
BP 70, 29280 Plouzané, France
(3) Pôle analytique des Eaux, technopôle Brest-lroise, 120 av. Alexis de Ronchon,
BP 52, 29280 Plouzané, France
A new fatty acid discovered from the scallop Pecten maximus was isolated
as a methyl ester using silver nitrate liquid chromatography and silicic
high performance liquid chromatography. This fatty acid was determined
to be a 22:4 geometrical isomer [A4?, 7?, 1 O?, 13?} (X) using gas chromatography-mass spectrometry analysis of their 2-alkenyl-4-4-dimethyloxazoline derivatives (GC/MS DMOX). The number of trans- double
bounds of this fatty acid was determined on silver ion high performance liquid chromatography by comparison with the mono-trans
geometrical isomers of 22:4 [A4cis, 7cis, 1 Ocis, 13cis] (S), obtained
through partial hydrazine reduction of all cis-22:6 (n-3) followed by a
p-toluenesulfinic isomerization of the derivatives. Comparing the four
trienes (22:3) obtained through partial hydrazine reduction of the two
compounds (X) and (S) determined that the trans-double bound was
located in Al 3.
This fatty acid identified as cis-4, 7, 10 trans-13-docosatetraenoic acid
has not been reportecl previously in any organisms and is believed to
be specific for the pectinid family.
107