9. SACCHARIDES: ALTERNATE ROUTES OF METABOLISM
477
TABLE VIII (Continued)
Organism
0
B. megatherium (4)
B. circulans
B. mesentericus
Schizosaccharomyces pombe (2)
Endomycopsis capsularis
Saccharomyces delbrueckii
Schwanniomyces occidentalis (2)
Debaryomyces hansenii
Hanseniaspora apiculata
Lipomyces lipoferus
Torulopsis holmii
Brettanomyces bruxellensis
Candida albicans
Kloeckera apiculata (2)
Neurospora sitophila
Nectria cinnabarina
Monascus ruber
Aspergillus nidulans
A. fumigatus
A. flavus
A. niger
Pencillium chrysogenum
Pencillium sp.
Pencillium sp.
Cercospora beticola
Helminthosporium
sativum
Monilia brunnea
Phoma betae
Dematium pullulans
Degree of
growth
6
+ + ( + + +)
+
+ +
+ +
+ + +
+ + +
+ + + +
+ + + +
+ + +
+ + + +
+ + +
+ + +
+ + + +
+ + (+ + +)
+ + +
+ +
+ + +
+ + +
+ + +
+ + +
+ + +
+ + +
+ + +
+ + +
+ +
+ + +
+
+ + +
+
Amount of
2-ketogluconate
decomposed
(%)
26-95
31
33
0-21
39
14
64-91
74
37
81
13
10
100
18-40
75
100
76
82
89
92
88
92
90
94
74
92
27
82
16
o 2
uptake
of
washed
cells
c
Ind.
Nt
0
Nt
Nt
0
Ind.
Ind.
0
Ind.
0
Nt
Ind.
0
Nt
Nt
Nt
Nt
Nt
Nt
Nt
Nt
Nt
Nt
Nt
Nt
Nt
Nt
Nt
Formation
δ-phosphate by
cell-free
extract**
+ +
Nt
—
Nt
Nt
Nt
—
—
Nt
—
Nt
Nt
—
Nt
—
Nt
—
Nt
—
—
Nt
—
—
Nt
Nt
Nt
Nt
—
Nt
a The number in parentheses after the names of organisms gives the number of strains
tested; if the name is not followed by a number in parentheses, only one strain was
tested.
b Degree of growth: + + + + , excellent; + + +, good; ++, moderate; +, weak.
c Oxygen uptake of washed cells: 0, no uptake; Nt, not tested; Ind., only after induction period.
d Intensity of spot on paper chromatograms of 2-keto-D-gluconate-6-phosphate
quinoxaline, after 3-hour enzyme treatment (= activity of cell-free extracts): + + +,
very intense, nearly complete phosphorylation; ++, intense, excellent phosphorylation;
+ , weak phosphorylation, + very weak phosphorylation but with a definite enzyme
activity; —, no enzyme activity detectable after 3 hours.