TABLE
XI
PHYSICOCHEMICAL PROPERTIES OF a-AMYLASES
(J.J
00
o
Pig pancreas (71)
0.6
3.1
5.2-5.6
Human pancreas (74-)
0.3
3.2
5.2-5.6
Human saliva (66)
0.3
3.2
5.2-5.6
Barley malt (129,
130)
15
3.1
5.7
B. subtilis (189)
6
2.4-3.0 a
A. oryzae (170)
10
4.2
4.2
Source of
a-amylase
Solubility in
H20 at
2°C.
pH 7.0
(gm./l00 ml.)
Electrophoretic
mobility
at pH 7.9
J.L
=
0.1
(cIn. 2
'
volt- 1
sec.- I
X 10- 5
)
Isoelectric
point
Diffusion
constant
D 20
X 10
7
(cm. 2
sec.-I)
8.05 (191)
6.53 (192)
Sedimentation
constant:
S
4.50 (191)
4.52 (192)
5.4
Molecular
weight c
45,000 (191)
59,500 (129)b,
54,000 (192)
Dispersion
constant,
Ao
(190)
243
271
~
~
I ~
tIj
b
a
pH 8.55.
b
From osmotic pressure measurements.
c
From sedimentation data.
XI
PHYSICOCHEMICAL PROPERTIES OF a-AMYLASES
(J.J
00
o
Pig pancreas (71)
0.6
3.1
5.2-5.6
Human pancreas (74-)
0.3
3.2
5.2-5.6
Human saliva (66)
0.3
3.2
5.2-5.6
Barley malt (129,
130)
15
3.1
5.7
B. subtilis (189)
6
2.4-3.0 a
A. oryzae (170)
10
4.2
4.2
Source of
a-amylase
Solubility in
H20 at
2°C.
pH 7.0
(gm./l00 ml.)
Electrophoretic
mobility
at pH 7.9
J.L
=
0.1
(cIn. 2
'
volt- 1
sec.- I
X 10- 5
)
Isoelectric
point
Diffusion
constant
D 20
X 10
7
(cm. 2
sec.-I)
8.05 (191)
6.53 (192)
Sedimentation
constant:
S
4.50 (191)
4.52 (192)
5.4
Molecular
weight c
45,000 (191)
59,500 (129)b,
54,000 (192)
Dispersion
constant,
Ao
(190)
243
271
~
~
I ~
tIj
b
a
pH 8.55.
b
From osmotic pressure measurements.
c
From sedimentation data.