TABLE I
REACTIONS AND ENZYMES INVOLVED IN THE EMBDEN-MEYERHOF SCHEME OF GLYCOLYSIS
bo
Reaction
Substrate (s)
Product (s)
Enzyme
1
la
lb
2
3
4
5
6
9
10
11
Glucose + ATP
Glycogen + P
t · <
Glucose-1-phosphate <
Glucose-6-phosphate <
Fructose-6-phosphate + ATP -
Fructose-l,6-diphosphate <
Dihydroxyacetone-1-phosphate <
Glyceraldehyde-3-phosphate
+ Pi + DPN+ <
-> Glucose-6-phosphate + ADP
-» Glucose-1-phosphate
-»Glucose-6-phosphate
->Fructose-6-phosphate
-> Fructose-1.6-diphosphate + ADP
-> Glyceraldehyde-3-phosphate +
dihydroxyacetone-1-phosphate
-> Glyceraldehyde-3-phosphate
1,3-Diphosphoglycerate + ADP <
3-Phosphoglycerate <
2-Phosphoglycerate <
Phosphoenol pyruvate + ADP <
Pyruvate + DPNH <
► 1,3-diphosphogly cerate + DPNH
► 3-phosphoglycerate + ATP
► 2-Phosphoglycerate
► Phosphoenol pyruvate
► Pyruvate + ATP
► Lactate + DPN+
Hexokinase
Phosphorylase
Phosphoglucomutase
Phosphoglucose isomerase
Phosphofructokinase
Aldolase
Triosephosphate isomerase
Glyceraldehyde-3-phosphatedehydrogenase
Phosphoglyceric phosphokinase
Phosphoglyceric mutase
Enolase
Pyruvate kinase
Lactic dehydrogenase
2
O
s
>
w
>
ATP balance:
Glycolysis of glucose
2 ATP
>2 ADP
4 ADP
>4ATP
Net balance: + 2 ATP
2 p.
:
2 DPN+
2 DPNH
► 1,3 Diphosphoglyceric acid
► 2 DPNH
► 2 DPN+
(Reactions 1 and 3)
(Reactions 7 and 10)
(Reaction 6)
(Reaction 6)
(Reaction 11)
Glycolysis of glycogen
1 ATP
► 1 ADP
4 ATP
> 4 ADP
Net balance: + 3 ATP
7
8