TABLE IX
METABOLIC REACTIONS OF ORGANIC SUBSTANCES IN WHICH HYDROGENASE IS INVOLVED
Reactions
Methane formation
Formic-hydrogen lyase
system
Phosphoroclastic
reaction
Reduction of aldehydes
and acids
Reductive deamination
of amino acids
Reduction of purines
Reduction of fumarate
C0
2 + 4H
2 -* CH
4 + 2H
2 0
CO + 3H
2 -» CH
4 + H
2 0
C0
2 + H
2 ^± HCOOH
Pyruvate + Pi ^ Acetyl phosphate + C0
2 + H
2
H
2 + Acetaldehyde —> EthanoJ
4H
2 + 2C0
2 -> CH
3 · COOH + 2H
2 0
H
2 + R · CH(NH
2 ) · COOH ->
NH
3 + R · CH
2 · COOH
H
2 + Xanthine ^± Hypoxanthine
H
2 + Fumarate ^ Succinate
Organisms
Methane bacteria (Methanobacterium omelianskii, M. formicicium,
Methanosarcina
barkerii, Methanococcus vannielii);
M. barkerii
Colon-aerogenes bacteria (Escherichia coli,
Aerobacter aerogenes, A. cloacae)
Clostridium butylicum, C. acetobutylicum and
other clostridia;
Desulfovibrio desulfuricans;
Micrococcus lactilyticus
Clostridium kluyveri
Clostridium aceticum
Clostridium sporcgenes
Micrococcus lactilyticus
Proteus vulgaris
E. coli
References
0
(0)
(&)
(c, a, e)
(f,g)
(A)
ω
(j)
(k)
(0
(m)
(ft)
(0)
«REFERENCES: (a) H. A. Barker, Proc. Natl. Acad. Sei. U. S. 29, 184 (1943); (6) A. J. Kluyver and C. G. T. P. Schnellen, Arch.
Biochem. 14, 57 (1947); (c) M. Stephenson and L. H. Stickland, Biochem. J. 26, 712 (1931); (d) M. Stephenson, Ergeb. Enzymforsch. 6,
139 (1937); (e) E. J. Ordal and H. O. Halvorson, J. Bacteriol. 38, 199 (1939); (/) H. J. Koepsell and M. J. Johnson, J. Biol. Chem. 145,
379 (1942); (g) J. Wilson, L. 0. Krampitz, and C. H. Werkman, Biochem. J. 42, 598 (1948); (A) J. C. Senez, Bull. soc. chim. biol. 36, 541
(1954); (i) A. T. Johns, /. Gen. Microbiol. 6, 326 (1951); (j) E. R. Stadtman and H. A. Barker, /. BioL Chem. 180, 1117 (1949); (k)
M. F. Utter and H. G. Wood, Advances in Enzymol. 12, 41 (1951); (/) J. G. Hoogerheide and W. Kocholaty, Biochem. J. 32, 949 (1938);
(m) H. R. Whiteley and H. C. Douglas, /. Bacteriol. 61, 605 (1951); (n) L. Farkas and E. Fischer, /. BioL Chem. 167, 787 (1947); (0)
J. Lascelles and J. L. Still, Proc. Linnean Soc. N. S. W. 72, 49 (1947).
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METABOLIC REACTIONS OF ORGANIC SUBSTANCES IN WHICH HYDROGENASE IS INVOLVED
Reactions
Methane formation
Formic-hydrogen lyase
system
Phosphoroclastic
reaction
Reduction of aldehydes
and acids
Reductive deamination
of amino acids
Reduction of purines
Reduction of fumarate
C0
2 + 4H
2 -* CH
4 + 2H
2 0
CO + 3H
2 -» CH
4 + H
2 0
C0
2 + H
2 ^± HCOOH
Pyruvate + Pi ^ Acetyl phosphate + C0
2 + H
2
H
2 + Acetaldehyde —> EthanoJ
4H
2 + 2C0
2 -> CH
3 · COOH + 2H
2 0
H
2 + R · CH(NH
2 ) · COOH ->
NH
3 + R · CH
2 · COOH
H
2 + Xanthine ^± Hypoxanthine
H
2 + Fumarate ^ Succinate
Organisms
Methane bacteria (Methanobacterium omelianskii, M. formicicium,
Methanosarcina
barkerii, Methanococcus vannielii);
M. barkerii
Colon-aerogenes bacteria (Escherichia coli,
Aerobacter aerogenes, A. cloacae)
Clostridium butylicum, C. acetobutylicum and
other clostridia;
Desulfovibrio desulfuricans;
Micrococcus lactilyticus
Clostridium kluyveri
Clostridium aceticum
Clostridium sporcgenes
Micrococcus lactilyticus
Proteus vulgaris
E. coli
References
0
(0)
(&)
(c, a, e)
(f,g)
(A)
ω
(j)
(k)
(0
(m)
(ft)
(0)
«REFERENCES: (a) H. A. Barker, Proc. Natl. Acad. Sei. U. S. 29, 184 (1943); (6) A. J. Kluyver and C. G. T. P. Schnellen, Arch.
Biochem. 14, 57 (1947); (c) M. Stephenson and L. H. Stickland, Biochem. J. 26, 712 (1931); (d) M. Stephenson, Ergeb. Enzymforsch. 6,
139 (1937); (e) E. J. Ordal and H. O. Halvorson, J. Bacteriol. 38, 199 (1939); (/) H. J. Koepsell and M. J. Johnson, J. Biol. Chem. 145,
379 (1942); (g) J. Wilson, L. 0. Krampitz, and C. H. Werkman, Biochem. J. 42, 598 (1948); (A) J. C. Senez, Bull. soc. chim. biol. 36, 541
(1954); (i) A. T. Johns, /. Gen. Microbiol. 6, 326 (1951); (j) E. R. Stadtman and H. A. Barker, /. BioL Chem. 180, 1117 (1949); (k)
M. F. Utter and H. G. Wood, Advances in Enzymol. 12, 41 (1951); (/) J. G. Hoogerheide and W. Kocholaty, Biochem. J. 32, 949 (1938);
(m) H. R. Whiteley and H. C. Douglas, /. Bacteriol. 61, 605 (1951); (n) L. Farkas and E. Fischer, /. BioL Chem. 167, 787 (1947); (0)
J. Lascelles and J. L. Still, Proc. Linnean Soc. N. S. W. 72, 49 (1947).
00
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o
o
w
o
>
52!
>
8
CD
00
-a