26
THOMAS S. ARGYRIS
the gradual loss of organ mass can be effective in initiating growth of the
organ concerned is also supported by the fact that the gradual loss of
tissue mass in chronic disease is associated with an increased proliferative
response on the part of the remaining organ (Crane and Dutta, 1963;
Hinman, 1926; Meader, 1963).
Thus, all the evidence taken as a whole demonstrates that the use of
Ehrlich ascites tumor invasion to remove skin mass is a legitimate
method, and that the loss of skin mass produced by the invading tumor
is not a sufficient cue for the stimulation of growth of resting hair follicles.
It is, therefore, quite likely that the loss of mass in wound healing also is
not in itself a sufficient stimulus for the induction of growth of the resting
hair follicles surrounding the wound. We must next determine if the loss
of skin mass can initiate epidermal and connective tissue growth.
The use of the Ehrlich ascites tumor as a means for removing epidermal
tissue was originally clouded by the fact that when the tumor invades the
subcutis of the skin it mildly stimulates the growth of the overlying
epidermis (T. S. Argyris and B. F. Argyris, 1962b). Once the tumor has
completely invaded and replaced the skin, there is no further proliferation
of the epidermis adjacent to the ulcerated area. Figure 9 shows a portion
of the epidermis adjacent to the ulcer produced by Ehrlich ascites tumor
invasion. It is thicker than normal. However the epidermal stimulation is
much less than that seen adjacent to a wound and can easily be distinguished from it (compare Figs. 1 and 9). Although we feel that this
suggests that the actual replacement of the epidermis by the tumor does
not result in stimulation of growth of the adjacent epidermis, the fact
that there is an initial mild epidermal stimulation by the invading tumor
has not permitted us to draw firm conclusions. Recently we have found a
tumor, the leukemia C-1498, that does not stimulate the overlying
epidermis as it invades the skin. Therefore, we (Argyris, 1966a) have
inoculated a series of mice with the C-1498 to determine if epidermal
proliferation occurs following complete replacement of the epidermis by
the tumor. Figure 11 shows a section of the skin that has been invaded
and replaced by the C-1498 tumor. No epidermal proliferation adjacent
to the area invaded by the tumor is seen.
Similar problems to those we have had with the epidermis have arisen
in evaluating the response of the connective tissue of the subcutis to the
invading Ehrlich ascites tumor (T. S. Argyris and B. F. Argyris, 1962b),
since the invading tumor mildly stimulates the connective tissue. By using
the C-1498 we have shown clearly that the removal of a portion of the
THOMAS S. ARGYRIS
the gradual loss of organ mass can be effective in initiating growth of the
organ concerned is also supported by the fact that the gradual loss of
tissue mass in chronic disease is associated with an increased proliferative
response on the part of the remaining organ (Crane and Dutta, 1963;
Hinman, 1926; Meader, 1963).
Thus, all the evidence taken as a whole demonstrates that the use of
Ehrlich ascites tumor invasion to remove skin mass is a legitimate
method, and that the loss of skin mass produced by the invading tumor
is not a sufficient cue for the stimulation of growth of resting hair follicles.
It is, therefore, quite likely that the loss of mass in wound healing also is
not in itself a sufficient stimulus for the induction of growth of the resting
hair follicles surrounding the wound. We must next determine if the loss
of skin mass can initiate epidermal and connective tissue growth.
The use of the Ehrlich ascites tumor as a means for removing epidermal
tissue was originally clouded by the fact that when the tumor invades the
subcutis of the skin it mildly stimulates the growth of the overlying
epidermis (T. S. Argyris and B. F. Argyris, 1962b). Once the tumor has
completely invaded and replaced the skin, there is no further proliferation
of the epidermis adjacent to the ulcerated area. Figure 9 shows a portion
of the epidermis adjacent to the ulcer produced by Ehrlich ascites tumor
invasion. It is thicker than normal. However the epidermal stimulation is
much less than that seen adjacent to a wound and can easily be distinguished from it (compare Figs. 1 and 9). Although we feel that this
suggests that the actual replacement of the epidermis by the tumor does
not result in stimulation of growth of the adjacent epidermis, the fact
that there is an initial mild epidermal stimulation by the invading tumor
has not permitted us to draw firm conclusions. Recently we have found a
tumor, the leukemia C-1498, that does not stimulate the overlying
epidermis as it invades the skin. Therefore, we (Argyris, 1966a) have
inoculated a series of mice with the C-1498 to determine if epidermal
proliferation occurs following complete replacement of the epidermis by
the tumor. Figure 11 shows a section of the skin that has been invaded
and replaced by the C-1498 tumor. No epidermal proliferation adjacent
to the area invaded by the tumor is seen.
Similar problems to those we have had with the epidermis have arisen
in evaluating the response of the connective tissue of the subcutis to the
invading Ehrlich ascites tumor (T. S. Argyris and B. F. Argyris, 1962b),
since the invading tumor mildly stimulates the connective tissue. By using
the C-1498 we have shown clearly that the removal of a portion of the