P R O T E I N S I N D E V E L O P M E N T
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FIG. 12. Antigens of toads and of their crosses. The different developmental stages are
shown on the ordinate in sketches and by stage numbers (following Rugh). B x B : Bufo
bufo; V x V : Bufo viridis; V x B : non-viable cross of B. viridis $ x B. bufo <$; Β x V :
viable cross of B. bufo $ x f i . viridis <$. The numbers on the abscissa represent the percentage ammonium sulphate saturation corresponding to concentrations between which it is
possible to precipitate the antigens. The precipitation with anti-Bufo bufo serum is shown
in the uninterrupted column. The precipitation with anti-Bufo viridis serum is shown in the
interrupted column. The arrow a represents the start of the anti-actin precipitation; the
arrow m the start of anti-myosin precipitation; the arrows p and r the start, in our experiments, of precipitation with anti-plasma and anti-red cells. (Some antigens of serum
proteins were found by Cooper (1946) in the egg.)