S U B J E C T I N D E X
771
Perchloric acid,
and inducing power o f ribonucleoproteins, 552
Performic acid,
and inducing agent, 550
Perfusion methods,
antibiotics in, 38
chambers for, 38
effects of, 38
for lens, 80
and maintenance o f stable concentrations, 13
of organ cultures, 80
for organs, 7, 80
Periblast tissue,
in fish embryos, 582
Pericardium,
hyaluronic acid produced by, 369
Perichondrium,
enzymes in, 648
Periderm,
on chick-skin, 663
vitamin A and hydrocortisone, on, 614
Periodate,
and inhibition o f aggregation, 500-1
Periodic acid-Schiff ( P A S ) ,
and chondroitin sulphate, 371
and mucins, 358-9, 666
positive cells and gonadotrophins, 606
positive m u c o i d , and mesenchymal
induction, 479
staining o f b o n e matrix, 646
Periosteum, 157, 597
Peripolesis, 195
Peritoneal cavity,
as culture chamber, 532
Permeability (see Cell permeability)
Peroxidase,
uptake by monocytes, 311
Peroxides,
as cause o f oxygen toxicity, 260
Perspex,
chamber for organ cultures, 76-7
as substratum for cells, 32
Petri dish,
for cell culture, 61
for culture o f ova, 583
for organ culture, 7 3 - 4
for re-aggregation, 492
p H ,
on carbohydrate metabolism, 258
and cell exudates, 494
BB*
at cell surface, 310
and collagen-fibre formation, 689
and culture o f lens, 80
and culture o f ova, 576-7
for cultures, 3, 2 3 - 5 , 27
for dissociating cells, 52
effect of, on mucopolysaccharides, 360,
362, 373, 375
and embryonic tissues, 566
in flasks, 41
glycolysis at acid, 266
local, 24
of medium, in cell cultures, 61, 375
and mucin clot, 366-7
and Naegleria, 166
and neuralization, 543
in organ cultures, 74
and oxygen consumption, 252
and protease o f chondrocytes, 381,
678-9
and protease o f monocytes, 310
and proteolysis in embryo juice, 310
regulation of, 23
and type o f metabolism, 254
Phagocytosis,
b y amoebocytes, 161
b y macrophages, 166
of pigment from marker cells, 517
vitamin C and, 686
Phase contrast microscope,
application to tissue culture, 12
hanging-drop cultures and, 33
slide chambers and, 38
Phenol red,
concentration, in aggregates o f nephric cells, 507
as indicator in media, 24
Phenylalanine,
analogues of, and growth, 572
cell strains and, 114
in Eagle's medium, 280
essential for heart tissue, 300
essential for tibia, 301
hydroxylation of, b y liver, 279
inhibiting pigment formation, 306
substitutes for, 300
and tyrosine, 289, 300
Phenylenediamine,
and oxygen consumption, 252
Phenylphosphate, 307
Phenylthiourea,
and pigment formation, 644
771
Perchloric acid,
and inducing power o f ribonucleoproteins, 552
Performic acid,
and inducing agent, 550
Perfusion methods,
antibiotics in, 38
chambers for, 38
effects of, 38
for lens, 80
and maintenance o f stable concentrations, 13
of organ cultures, 80
for organs, 7, 80
Periblast tissue,
in fish embryos, 582
Pericardium,
hyaluronic acid produced by, 369
Perichondrium,
enzymes in, 648
Periderm,
on chick-skin, 663
vitamin A and hydrocortisone, on, 614
Periodate,
and inhibition o f aggregation, 500-1
Periodic acid-Schiff ( P A S ) ,
and chondroitin sulphate, 371
and mucins, 358-9, 666
positive cells and gonadotrophins, 606
positive m u c o i d , and mesenchymal
induction, 479
staining o f b o n e matrix, 646
Periosteum, 157, 597
Peripolesis, 195
Peritoneal cavity,
as culture chamber, 532
Permeability (see Cell permeability)
Peroxidase,
uptake by monocytes, 311
Peroxides,
as cause o f oxygen toxicity, 260
Perspex,
chamber for organ cultures, 76-7
as substratum for cells, 32
Petri dish,
for cell culture, 61
for culture o f ova, 583
for organ culture, 7 3 - 4
for re-aggregation, 492
p H ,
on carbohydrate metabolism, 258
and cell exudates, 494
BB*
at cell surface, 310
and collagen-fibre formation, 689
and culture o f lens, 80
and culture o f ova, 576-7
for cultures, 3, 2 3 - 5 , 27
for dissociating cells, 52
effect of, on mucopolysaccharides, 360,
362, 373, 375
and embryonic tissues, 566
in flasks, 41
glycolysis at acid, 266
local, 24
of medium, in cell cultures, 61, 375
and mucin clot, 366-7
and Naegleria, 166
and neuralization, 543
in organ cultures, 74
and oxygen consumption, 252
and protease o f chondrocytes, 381,
678-9
and protease o f monocytes, 310
and proteolysis in embryo juice, 310
regulation of, 23
and type o f metabolism, 254
Phagocytosis,
b y amoebocytes, 161
b y macrophages, 166
of pigment from marker cells, 517
vitamin C and, 686
Phase contrast microscope,
application to tissue culture, 12
hanging-drop cultures and, 33
slide chambers and, 38
Phenol red,
concentration, in aggregates o f nephric cells, 507
as indicator in media, 24
Phenylalanine,
analogues of, and growth, 572
cell strains and, 114
in Eagle's medium, 280
essential for heart tissue, 300
essential for tibia, 301
hydroxylation of, b y liver, 279
inhibiting pigment formation, 306
substitutes for, 300
and tyrosine, 289, 300
Phenylenediamine,
and oxygen consumption, 252
Phenylphosphate, 307
Phenylthiourea,
and pigment formation, 644