754
S U B J E C T I N D E X
Hormones—continued
on carbohydrate metabolism, 2 6 4 - 6 ,
650
and cell lines, 284, 293
and differentiation, 469, 650
early use in media, 102
male, 581
and mammary gland, 6 3 3 - 9 , 650
and mammary tumours, 6 3 8 - 9
in natural media, 606, 634
sensitivity to, 592
in synthetic media, 116
tissue culture and, 12-13, 592, 650
in vitro and in vivo, 13
Humerus,
thyroxine and, 641
vitamin A and, 674
Hyalobionic acid,
and chondroitin sulphate, 364
Hyaluronic acid,
ascorbic acid and, 690
and cell surface, 376
and corticosteroids, 607
and embryo extract, 366, 383
estimation of, in tissue cultures, 366,369
and fibroblasts, 374
hydrolysis of, 364
in mast cells, 358
and metachromacy, 358-9
and " m u c i n " clot, 366
precursors of, 378
production in tissue cultures, 366, 3 6 8 -
70, 384
properties and distribution of, 356
separation of, 361-2
in suspension cultures, 69
and synovial cells, 368, 372, 375
and turbidity with protein, 367, 369
turnover of, 380
in Walker tumour, 256, 382-3
Hyaluronidase,
action o n mast cells, 358
and basophilia, 382
and chondroitin sulphate, 361
and dispersion o f cells, 56-7
and invasiveness o f tumours, 382-3
on skeletal rudiments in vitro, 371,382
in tissue-culture media, 366
Hydrochloric acid,
dorsalizing action of, 551
Hydrocortisone,
o n cartilage and bone, 381, 6 1 2 - 1 5
and lymphocytes, 6 0 8 - 9 , 612
and lysosomes, 611-12
and mammary gland, 6 3 5 - 7
and respiration, 607
on skin, 6 1 0 - 1 1 , 614
and uptake o f sulphate, 385
vitamin A and, 614-15
Hydrolases,
lysosomes and, 380, 384, 679
Hydrogen peroxide,
ascorbic acid and, 682, 689
and differentiation o f somites, 570
and hydroxylation o f proline, 689
Hydrogen ion concentration ( ^ p H ) , 2 3 - 5
Hydrogen sulphide,
and differentiation o f somites, 5 6 8 - 9
Hydroxylamine,
and centromere regions, 446
Hydroxylation, 690
Hydroxyproline,
and bone cells, 163
and collagen, 370, 689
and growth o f carrot tissues, 163
and heart tissue, 299
from labelled proline, 305-7, 689
and osteoblasts, 305
vitamin A and, 310
Hyperplasia,
of prostatic epithelium, 628-31
Hyperblastulae,
from fish eggs, 582
Hyperglycaemic factor ( H G F ) ,
and bone growth, 599
Hypophysis, see Pituitary gland
Hypothalamic tissue,
and pituitary, 602, 604
Hypotonic solutions,
and cartilage, 381
for dispersing chromosomes, 12, 33,402
I
Identification o f cells, 143-74
in cell aggregates, 507, 517
in cell cultures, 62
cinephotography and, 12
in formation o f clones, 66
in hanging-drop cultures, 37
nuclei and, 173
Idiogram (human), 4 0 4 - 5
of hermaphrodites, 408
of mongoloid, 410
of Turner's syndrome, 407
S U B J E C T I N D E X
Hormones—continued
on carbohydrate metabolism, 2 6 4 - 6 ,
650
and cell lines, 284, 293
and differentiation, 469, 650
early use in media, 102
male, 581
and mammary gland, 6 3 3 - 9 , 650
and mammary tumours, 6 3 8 - 9
in natural media, 606, 634
sensitivity to, 592
in synthetic media, 116
tissue culture and, 12-13, 592, 650
in vitro and in vivo, 13
Humerus,
thyroxine and, 641
vitamin A and, 674
Hyalobionic acid,
and chondroitin sulphate, 364
Hyaluronic acid,
ascorbic acid and, 690
and cell surface, 376
and corticosteroids, 607
and embryo extract, 366, 383
estimation of, in tissue cultures, 366,369
and fibroblasts, 374
hydrolysis of, 364
in mast cells, 358
and metachromacy, 358-9
and " m u c i n " clot, 366
precursors of, 378
production in tissue cultures, 366, 3 6 8 -
70, 384
properties and distribution of, 356
separation of, 361-2
in suspension cultures, 69
and synovial cells, 368, 372, 375
and turbidity with protein, 367, 369
turnover of, 380
in Walker tumour, 256, 382-3
Hyaluronidase,
action o n mast cells, 358
and basophilia, 382
and chondroitin sulphate, 361
and dispersion o f cells, 56-7
and invasiveness o f tumours, 382-3
on skeletal rudiments in vitro, 371,382
in tissue-culture media, 366
Hydrochloric acid,
dorsalizing action of, 551
Hydrocortisone,
o n cartilage and bone, 381, 6 1 2 - 1 5
and lymphocytes, 6 0 8 - 9 , 612
and lysosomes, 611-12
and mammary gland, 6 3 5 - 7
and respiration, 607
on skin, 6 1 0 - 1 1 , 614
and uptake o f sulphate, 385
vitamin A and, 614-15
Hydrolases,
lysosomes and, 380, 384, 679
Hydrogen peroxide,
ascorbic acid and, 682, 689
and differentiation o f somites, 570
and hydroxylation o f proline, 689
Hydrogen ion concentration ( ^ p H ) , 2 3 - 5
Hydrogen sulphide,
and differentiation o f somites, 5 6 8 - 9
Hydroxylamine,
and centromere regions, 446
Hydroxylation, 690
Hydroxyproline,
and bone cells, 163
and collagen, 370, 689
and growth o f carrot tissues, 163
and heart tissue, 299
from labelled proline, 305-7, 689
and osteoblasts, 305
vitamin A and, 310
Hyperplasia,
of prostatic epithelium, 628-31
Hyperblastulae,
from fish eggs, 582
Hyperglycaemic factor ( H G F ) ,
and bone growth, 599
Hypophysis, see Pituitary gland
Hypothalamic tissue,
and pituitary, 602, 604
Hypotonic solutions,
and cartilage, 381
for dispersing chromosomes, 12, 33,402
I
Identification o f cells, 143-74
in cell aggregates, 507, 517
in cell cultures, 62
cinephotography and, 12
in formation o f clones, 66
in hanging-drop cultures, 37
nuclei and, 173
Idiogram (human), 4 0 4 - 5
of hermaphrodites, 408
of mongoloid, 410
of Turner's syndrome, 407