750
S U B J E C T I N D E X
Glutathione (GSH)—continued
and redox potentials, 687
and stability o f ascorbic acid, 6 8 3 - 4
as substitute for cystine, 300
Glycerol,
and freezing o f tissues, 28
Glycine,
and amino-acid formation, 288, 295
and b o n e cells, 163, 299, 304
in collagen, 370
continued culture, and uptake of, 308
and culture o f ova, 576
from glucose, 250, 283, 287, 299
and heart tissue, 299, 303
incorporation o f radioactive, 570, 572
for isolated cells, 63
and kidney, 303
as link in glycoproteins, 365
and liver, 303
in metabolic pool, 570
and nucleic acids, 300
oestradiol and uptake of, 306, 308
precursors of, 287-8
and prostate, 304
Glycogen,
and adipose tissue, 595-7
in H e L a cells, 254
hydrolysis by embryo extract, 247
as inducing agent, 543
insulin and, 595, 597, 599, 600
in liver cells, 170, 254, 507
separation from mucopolysaccharides,
361
synthesis and turnover, 242
Glycolysis,
aerobic, 250, 267
and A T P formation, 242-3
in bone cells, 648
and cell division, 256
in cell strains, 266
corticosteroids and, 265, 615
of cultured cells, 248, 250, 254, 375
of embryonic tissues, 70
galactose and, 247
glucose concentration and, 259
and growth, 2 5 4 - 5 , 257, 2 6 1 - 2
and heart, 571
insulin and, 2 6 4 - 5 , 267
in L-cells, and hydrocortisone, 607
metabolic inhibitors and, 253
in normal and malignant cells, 594
p H and, 258, 266
phosphate and, 111
in poorly oxygenated cultures, 24, 70,251
and respiration, 2 5 1 - 3 , 262, 375
thyroid and, 2 6 5 - 6
Glycoproteins,
analysis of, 365
definition of, 354-5
glycine and formation of, 365
and spreading o f cells, 32, 63
in thyroid cells, 477
Goblet cells,
and polysaccharides, 26
and sulphate, 26
in trachea, 160
vitamin A and production of, 664, 671
Golgi apparatus ( b o d y ) ,
form of, 161,
vitamin C and, 681
Gonadotrophin, 6 0 1 - 6
dependent on other hormones, 606
on ovaries in synthetic medium, 605
Gonads,
amino-acid requirements of, 300-1
in cell aggregates, 509
entry o f germ cells into, 565
region o f embryo producing, 537
resulting from fused embryos, 581
sex hormones and development of,
617-18
Gonocytes (germ cells),
origin o f primary, 565
transport of, 565
Gradient,
animal-vegetal, 161
of cell density, 563
of embryo-forming capacity, 563
-field, chordo-mesoderm as, 535
o f growth potential, 563
of induction, 5 4 6 - 9
of metabolites, in aggregates, 523
of morphogenetic potentialities, 563
Gradient-slide method, 45
Grafts,
on chorioallantois, 4 7 1 - 2 , 578
of embryonic tissues, 557
Granules,
cytoplasmic, 152
Granuloma (carrageenan),
ascorbic acid and collagen formation in,
688-90
Grid methods,
for organ culture, 77-9
S U B J E C T I N D E X
Glutathione (GSH)—continued
and redox potentials, 687
and stability o f ascorbic acid, 6 8 3 - 4
as substitute for cystine, 300
Glycerol,
and freezing o f tissues, 28
Glycine,
and amino-acid formation, 288, 295
and b o n e cells, 163, 299, 304
in collagen, 370
continued culture, and uptake of, 308
and culture o f ova, 576
from glucose, 250, 283, 287, 299
and heart tissue, 299, 303
incorporation o f radioactive, 570, 572
for isolated cells, 63
and kidney, 303
as link in glycoproteins, 365
and liver, 303
in metabolic pool, 570
and nucleic acids, 300
oestradiol and uptake of, 306, 308
precursors of, 287-8
and prostate, 304
Glycogen,
and adipose tissue, 595-7
in H e L a cells, 254
hydrolysis by embryo extract, 247
as inducing agent, 543
insulin and, 595, 597, 599, 600
in liver cells, 170, 254, 507
separation from mucopolysaccharides,
361
synthesis and turnover, 242
Glycolysis,
aerobic, 250, 267
and A T P formation, 242-3
in bone cells, 648
and cell division, 256
in cell strains, 266
corticosteroids and, 265, 615
of cultured cells, 248, 250, 254, 375
of embryonic tissues, 70
galactose and, 247
glucose concentration and, 259
and growth, 2 5 4 - 5 , 257, 2 6 1 - 2
and heart, 571
insulin and, 2 6 4 - 5 , 267
in L-cells, and hydrocortisone, 607
metabolic inhibitors and, 253
in normal and malignant cells, 594
p H and, 258, 266
phosphate and, 111
in poorly oxygenated cultures, 24, 70,251
and respiration, 2 5 1 - 3 , 262, 375
thyroid and, 2 6 5 - 6
Glycoproteins,
analysis of, 365
definition of, 354-5
glycine and formation of, 365
and spreading o f cells, 32, 63
in thyroid cells, 477
Goblet cells,
and polysaccharides, 26
and sulphate, 26
in trachea, 160
vitamin A and production of, 664, 671
Golgi apparatus ( b o d y ) ,
form of, 161,
vitamin C and, 681
Gonadotrophin, 6 0 1 - 6
dependent on other hormones, 606
on ovaries in synthetic medium, 605
Gonads,
amino-acid requirements of, 300-1
in cell aggregates, 509
entry o f germ cells into, 565
region o f embryo producing, 537
resulting from fused embryos, 581
sex hormones and development of,
617-18
Gonocytes (germ cells),
origin o f primary, 565
transport of, 565
Gradient,
animal-vegetal, 161
of cell density, 563
of embryo-forming capacity, 563
-field, chordo-mesoderm as, 535
o f growth potential, 563
of induction, 5 4 6 - 9
of metabolites, in aggregates, 523
of morphogenetic potentialities, 563
Gradient-slide method, 45
Grafts,
on chorioallantois, 4 7 1 - 2 , 578
of embryonic tissues, 557
Granules,
cytoplasmic, 152
Granuloma (carrageenan),
ascorbic acid and collagen formation in,
688-90
Grid methods,
for organ culture, 77-9