S U B J E C T I N D E X
739
Cobalt,
and mucopolysaccharide production,
375
and the nucleolus, 217
requirement for, 111, 282
tolerance, 375
Cocarboxylase,
and cloning o f H e L a cells, 684
in medium T A C P I , 128
Coding triplets, 321
Cohesion,
affected b y trypsin, 58
calcium and, 51, 5 2 - 4 , 499-500
of epitheliocytes, 160
of intestinal cells, 538
lipoproteins and, 500
in re-aggregated cells, 69
Colchicine,
and chromosome maps, 402
and exchanges between chromatids,
427-8
and mitosis, 206, 212
Collagen,
ascorbic acid and, 685, 688-9
dermal fibroblasts and, 370
epitheliocytes and, 163
fibres and cell surface, 60
formation and amino acids, 304-5
and hyaluronic acid, 369
mechanocytes and, 163, 165
oriented, and cell movement, 187
in prostate, and oestrogens, 6 2 8 - 9
reconstituted, as substratum, 32, 44
resorption of, 310
and silicic acid, 305
and trypsin, 56
Collagenase, 56
and dissociation o f cells, 510
and uptake o f sulphate, 379
Colloid,
of thyroid, developed in vitro, 477, 601
thyrotrophin and, 6 0 1 - 3 , 606
Colloid osmotic pressure,
of proteins in tissue fluid, 23
Colloids,
in media for cell culture, 63
Colonies,
hanging-drop cultures as, 37
Concentration gradients,
and cell movement, 179, 181, 185, 196
maintenance of, 240, 244
Concentration o f media,
optimum versus physiological, 107
Conjunctiva,
and amino-acid incorporation, 331
carbohydrate sources for, 2 4 5 - 6
colonies of, 65
keratinization of, b y citral, 661
and mucopolysaccharide production,
377
and
population
dependent
metabolites, 286
pure strain of, 170
respiration of, 252
variant cell line of, 247, 377
Connective-tissue cells (see also Fibroblasts)
amino-acid requirements of, 114
and continuity o f ectoderm, 534
and continuity o f endoderm, 538
and differentiation, 6, 633
enzymes in, 648-9
hetero-afHnities of, 522
in heterotypic-interspecific aggregates,
518
morphology of, 159
parathyroid and, 646-7
position of, in aggregates, 522
and response o f epithelia to hormones,
633-5
R N A and morphology of, 156
Contact (confronted) cultures, 601 etseq.,
650
between pituitary and adrenal cortex,
6 0 3 - 4
between pituitary and ovary, 6 0 5 - 6
between pituitary and testis, 606
between pituitary and thyroid, 602
Contact following, 197
Contact guidance, 187-8, 196-7, 523
Contact inhibition,
and cell degeneration, 189
of cell types, 189-90
and colony formation, 193-4
and directional movement, 196-7
between fibroblasts, 165, 184, 188-9,
191-4
and mitosis, 189, 504
and nature o f substrate, 493
and regionalization in aggregates,
523
and trapping o f cells, 191
Contact stimulation, 180, 184, 186-90
739
Cobalt,
and mucopolysaccharide production,
375
and the nucleolus, 217
requirement for, 111, 282
tolerance, 375
Cocarboxylase,
and cloning o f H e L a cells, 684
in medium T A C P I , 128
Coding triplets, 321
Cohesion,
affected b y trypsin, 58
calcium and, 51, 5 2 - 4 , 499-500
of epitheliocytes, 160
of intestinal cells, 538
lipoproteins and, 500
in re-aggregated cells, 69
Colchicine,
and chromosome maps, 402
and exchanges between chromatids,
427-8
and mitosis, 206, 212
Collagen,
ascorbic acid and, 685, 688-9
dermal fibroblasts and, 370
epitheliocytes and, 163
fibres and cell surface, 60
formation and amino acids, 304-5
and hyaluronic acid, 369
mechanocytes and, 163, 165
oriented, and cell movement, 187
in prostate, and oestrogens, 6 2 8 - 9
reconstituted, as substratum, 32, 44
resorption of, 310
and silicic acid, 305
and trypsin, 56
Collagenase, 56
and dissociation o f cells, 510
and uptake o f sulphate, 379
Colloid,
of thyroid, developed in vitro, 477, 601
thyrotrophin and, 6 0 1 - 3 , 606
Colloid osmotic pressure,
of proteins in tissue fluid, 23
Colloids,
in media for cell culture, 63
Colonies,
hanging-drop cultures as, 37
Concentration gradients,
and cell movement, 179, 181, 185, 196
maintenance of, 240, 244
Concentration o f media,
optimum versus physiological, 107
Conjunctiva,
and amino-acid incorporation, 331
carbohydrate sources for, 2 4 5 - 6
colonies of, 65
keratinization of, b y citral, 661
and mucopolysaccharide production,
377
and
population
dependent
metabolites, 286
pure strain of, 170
respiration of, 252
variant cell line of, 247, 377
Connective-tissue cells (see also Fibroblasts)
amino-acid requirements of, 114
and continuity o f ectoderm, 534
and continuity o f endoderm, 538
and differentiation, 6, 633
enzymes in, 648-9
hetero-afHnities of, 522
in heterotypic-interspecific aggregates,
518
morphology of, 159
parathyroid and, 646-7
position of, in aggregates, 522
and response o f epithelia to hormones,
633-5
R N A and morphology of, 156
Contact (confronted) cultures, 601 etseq.,
650
between pituitary and adrenal cortex,
6 0 3 - 4
between pituitary and ovary, 6 0 5 - 6
between pituitary and testis, 606
between pituitary and thyroid, 602
Contact following, 197
Contact guidance, 187-8, 196-7, 523
Contact inhibition,
and cell degeneration, 189
of cell types, 189-90
and colony formation, 193-4
and directional movement, 196-7
between fibroblasts, 165, 184, 188-9,
191-4
and mitosis, 189, 504
and nature o f substrate, 493
and regionalization in aggregates,
523
and trapping o f cells, 191
Contact stimulation, 180, 184, 186-90