S U B J E C T I N D E X
729
Albumin,
and culture o f o v e , 5 7 6 - 7 , 580, 583
density gradient of, 45
and development o f somites, 569
formation o f serum-, 306
in media for cell cultures, 63
in media for organ cultures, 75, 5 5 3 - 4 ,
565, 567
serum-, as supplement, 109
and spreading o f H e L a cells, 122
Alcian blue,
and mucopolysaccharides, 359
Alcohols,
and inducing agents, 550
and neuralization, 543
as sources o f carbohydrate, 245
Aldolase,
and enzymatic adaptation, 264
Aldosterone,
and N a / K , 26
Allantois,
blood vessels in, 579
Allergic reactions,
enzyme systems in, 310
Allocycly (see Heteropyknosis)
Amenorrhea,
chromosome pattern and, 414
and X X X condition, 407
Amethopterin,
and H e L a cells, 230
and synchronization o f mitosis, 429-31
Amines,
biologically active, production of, 2 9 3 - 4
A m i n o acids,
analogues of, and growth, 572
basic, as supplements, 109
and cartilage, 300
chromatography of, 3 0 3 - 4
concentration of, b y cells, 290-1
and culture o f blastoderms, 566
and differentiation, 470, 567-8
dose-response relationships of, 113
essential and non-essential, 113-16,
2 7 8 - 9 , 2 8 9 - 9 0 , 299-302
formation of, 2 5 4 - 5 , 2 8 7 - 9 , 295
from glucose, 112, 115, 2 8 7 - 8 , 2 9 9 - 3 0 0
from glutamine, 2 8 7 - 8
and gonads, 300-1
and heart, 299-300
and Hensen's node, 566
labelled, 331, 480
laevorotatory, 109
in media for organ culture, 75-6
metabolism of, 2 7 7 - 9 5 , 297-313
minimum for cell strains, 114
and nucleic acids, 300
pools, 290-1
and protein synthesis, 290, 321, 331
and pyridoxal, 116
as source o f energy, 249
-sparing effect o f glucose, 249
in synthetic media, 103, 107, 114
and syrinx, 300-1
and tibia, 299-301
toxicity of, 107
transport, 290-1
utilization of, 107-8, 128, 302-5
p- aminobenzoic acid,
for organ culture, 127, 128
in synthetic media, 115
a-amino group,
from glutamic acid, 288-9
Aminopterin,
and synchronization o f mitosis, 430
and thymine metabolism, 417
Aminosugars,
estimation of, 362-3
vitamin A and, in cartilage, 677
Amitosis, 216, 422
A m m o n i a ,
and formation o f glucosamine, 377
and induction, 543, 551
production of, and glucose, 249
A m m o n i u m ,
and Naegleria, 166
quaternary, and cell transformation,
167
quaternary,
and
precipitation
o f
heparin, 361
quaternary, and precipitation nucleic
acids, 361
A m n i o n cells (FL cells),
and hexosamine production, 375
labelling of, 327
Amniotic fluid,
and culture o f ova, 578
Amoebocytes,
behaviour of, o n fish scale, 152
conversion to fibroblasts, 166
definition of, 146-7
fusion of, 147, 164
and ionic balance, 167
and mechanocytes compared, 162, 166
movement of, 178-9
729
Albumin,
and culture o f o v e , 5 7 6 - 7 , 580, 583
density gradient of, 45
and development o f somites, 569
formation o f serum-, 306
in media for cell cultures, 63
in media for organ cultures, 75, 5 5 3 - 4 ,
565, 567
serum-, as supplement, 109
and spreading o f H e L a cells, 122
Alcian blue,
and mucopolysaccharides, 359
Alcohols,
and inducing agents, 550
and neuralization, 543
as sources o f carbohydrate, 245
Aldolase,
and enzymatic adaptation, 264
Aldosterone,
and N a / K , 26
Allantois,
blood vessels in, 579
Allergic reactions,
enzyme systems in, 310
Allocycly (see Heteropyknosis)
Amenorrhea,
chromosome pattern and, 414
and X X X condition, 407
Amethopterin,
and H e L a cells, 230
and synchronization o f mitosis, 429-31
Amines,
biologically active, production of, 2 9 3 - 4
A m i n o acids,
analogues of, and growth, 572
basic, as supplements, 109
and cartilage, 300
chromatography of, 3 0 3 - 4
concentration of, b y cells, 290-1
and culture o f blastoderms, 566
and differentiation, 470, 567-8
dose-response relationships of, 113
essential and non-essential, 113-16,
2 7 8 - 9 , 2 8 9 - 9 0 , 299-302
formation of, 2 5 4 - 5 , 2 8 7 - 9 , 295
from glucose, 112, 115, 2 8 7 - 8 , 2 9 9 - 3 0 0
from glutamine, 2 8 7 - 8
and gonads, 300-1
and heart, 299-300
and Hensen's node, 566
labelled, 331, 480
laevorotatory, 109
in media for organ culture, 75-6
metabolism of, 2 7 7 - 9 5 , 297-313
minimum for cell strains, 114
and nucleic acids, 300
pools, 290-1
and protein synthesis, 290, 321, 331
and pyridoxal, 116
as source o f energy, 249
-sparing effect o f glucose, 249
in synthetic media, 103, 107, 114
and syrinx, 300-1
and tibia, 299-301
toxicity of, 107
transport, 290-1
utilization of, 107-8, 128, 302-5
p- aminobenzoic acid,
for organ culture, 127, 128
in synthetic media, 115
a-amino group,
from glutamic acid, 288-9
Aminopterin,
and synchronization o f mitosis, 430
and thymine metabolism, 417
Aminosugars,
estimation of, 362-3
vitamin A and, in cartilage, 677
Amitosis, 216, 422
A m m o n i a ,
and formation o f glucosamine, 377
and induction, 543, 551
production of, and glucose, 249
A m m o n i u m ,
and Naegleria, 166
quaternary, and cell transformation,
167
quaternary,
and
precipitation
o f
heparin, 361
quaternary, and precipitation nucleic
acids, 361
A m n i o n cells (FL cells),
and hexosamine production, 375
labelling of, 327
Amniotic fluid,
and culture o f ova, 578
Amoebocytes,
behaviour of, o n fish scale, 152
conversion to fibroblasts, 166
definition of, 146-7
fusion of, 147, 164
and ionic balance, 167
and mechanocytes compared, 162, 166
movement of, 178-9