16. A C T I O N S OF H O R M O N E S
undifferentiated gonad was seen after addition of oestradiol benzoate
to the culture medium.
Wolff (1953) also demonstrated the role of testosterone in the regression of the male Miillerian ducts of the chick (Figs. 9 and 10). Male
ducts explanted after sexual differentiation usually regress in vitro, an
effect attributed to their previous exposure in vivo to male sex hormones.
FIG. 9. Development of the male Miillerian duct of the chick explanted before sexual differentiation and grown in vitro for 5 days. The duct is lined by a row of columnar epithelium
surrounded by healthy connective tissue. (From Wolff, 1953.)
FIG. 10. Comparable explant of male Miillerian duct grown with testosterone. Note necrosis
of epithelium and connective tissue and disappearance of lumen. (From Wolff, 1953.)
undifferentiated gonad was seen after addition of oestradiol benzoate
to the culture medium.
Wolff (1953) also demonstrated the role of testosterone in the regression of the male Miillerian ducts of the chick (Figs. 9 and 10). Male
ducts explanted after sexual differentiation usually regress in vitro, an
effect attributed to their previous exposure in vivo to male sex hormones.
FIG. 9. Development of the male Miillerian duct of the chick explanted before sexual differentiation and grown in vitro for 5 days. The duct is lined by a row of columnar epithelium
surrounded by healthy connective tissue. (From Wolff, 1953.)
FIG. 10. Comparable explant of male Miillerian duct grown with testosterone. Note necrosis
of epithelium and connective tissue and disappearance of lumen. (From Wolff, 1953.)