T A B L E I I I
Biosynthesis o f amino acids in mammalian cell cultures
(Eagle and Piez, 1962b)
A m i n o acid
% o f carbon skeleton deriving from uniformly
1 4
C - l a b e l l e d
% o f a - N H
2
deriving from
1 5
N - g l u t a m i c
synthesized
Glucose
Serine*
Glycine*
R i b o s e f
Pyruvatef
Glutamine*
Glutamic J
Arginine
acid
w
Alanine
71-101
0-4-1-4
0-2
0-8-4
85
7
7
-
80
A G L E Al
Serine
70-111
22-102
10
6 3 - 6 6
1-7
2
9-9
-
75
A G L E Al
Glycine
69-106
25-99
50
5 8 - 6 0
1-9
7-0
-
76
0
Asparagine
Aspartic
8-23
0-1-1-3
< 0 - l
0-48-12
4-7
71-87
100
-
78
L E O N
Glutamine
Glutamic
4-11
0-02-0-2
< 0 - l
0-31-0-63
4-1
101
84
-
83
L E V I
Proline
3-18
0-09-0-44
0-15
0-2-0-3
3-1
59
27
26
58
H
o
*In basal growth medium, containing glucose,
f i n medium lacking glucose.
{ I n medium lacking glutamine.
288
H A R R Y E A G L E A N D L E O N L E V I N T O W
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