7. C A R B O H Y D R A T E A N D E N E R G Y M E T A B O L I S M
253
Cell
Effect
or
•
Substance
Cone, (M)
tissue Growth resp.
Other
Reference
Antimycin A
(0-05 ^ g / m l )
L
_
4
Arsenite
io5
L
-
Glycolysis -
3
Azide
I O -
1
L
-
Glycolysis -
4
2 x 1 0 -
3
C C T
Mitosis 0
Migration -
5
2 x IO"
3
R C T
0
2
io4
L
-
Glycolysis 0
Migration -
Mitosis 0
3
Carbon m o n o x i d e
R C T
C C T
0
Glycolysis 0
Migration -
Mitosis 0
2
5
Cyanide
2 x 1 0 -
3
C C T
Migration -
Mitosis 0
5
io3
R C T
Death
2
io5
C C T ,
-
Cytolysis
1
R C T
Cytolysis
2:4-Dinitrophenol
io4
L
-
6
i o -
5
- i o -
4
L
+
6
I O - M O -
4
L
+
4
2 x 10~
4
R C T
0
2
Fluoride
io3
5 x 1 0 -
4
R C T
L
Death
2
6
Iodoacetamide
io3
R C T
Death
2
Iodoacetate
io5
L
_
6
Malonate
5 x 1 0 -
2
L
-
4
Abbreviations: L, strain L cell; C C T , chick connective tissue; R C T , rat connective
tissue; 0, no effect; - inhibition; -f, increase.
References: 1. Danes and Leinfelder, 1951; 2. Harris, 1956; 3. Pace and Elrod, 1960;
4. Packer, Papa, Rust and Ajl, 1959; 5. Pomerat and Willmer, 1939; 6. Siegel
and Cailleau, 1956.
that respiration could fluctuate widely, depending both on the conditions of culture and the conditions under which respiration was
measured. It should therefore be noted that they may not represent
maximum values.
Most authors have reported very low values for endogenous carbohydrate in cultured cells (Gill, 1938; Nirenberg, 1958; Peppers,
Westfall and Earle, 1959; Roussy and Craciun, 1928) but W u (1959)
falls within the above range and although each cell has its own
characteristic value this does not seem to be closely correlated with its
glycolytic capacity. On the whole it seems to be more closely related
to the amount of cytoplasm in the cell (Phillips et al., 1960). Most ofthe
results reported in Table I V were reported before it was appreciated
T A B L E V
Effect o f various substances o n growth and metabolism
253
Cell
Effect
or
•
Substance
Cone, (M)
tissue Growth resp.
Other
Reference
Antimycin A
(0-05 ^ g / m l )
L
_
4
Arsenite
io5
L
-
Glycolysis -
3
Azide
I O -
1
L
-
Glycolysis -
4
2 x 1 0 -
3
C C T
Mitosis 0
Migration -
5
2 x IO"
3
R C T
0
2
io4
L
-
Glycolysis 0
Migration -
Mitosis 0
3
Carbon m o n o x i d e
R C T
C C T
0
Glycolysis 0
Migration -
Mitosis 0
2
5
Cyanide
2 x 1 0 -
3
C C T
Migration -
Mitosis 0
5
io3
R C T
Death
2
io5
C C T ,
-
Cytolysis
1
R C T
Cytolysis
2:4-Dinitrophenol
io4
L
-
6
i o -
5
- i o -
4
L
+
6
I O - M O -
4
L
+
4
2 x 10~
4
R C T
0
2
Fluoride
io3
5 x 1 0 -
4
R C T
L
Death
2
6
Iodoacetamide
io3
R C T
Death
2
Iodoacetate
io5
L
_
6
Malonate
5 x 1 0 -
2
L
-
4
Abbreviations: L, strain L cell; C C T , chick connective tissue; R C T , rat connective
tissue; 0, no effect; - inhibition; -f, increase.
References: 1. Danes and Leinfelder, 1951; 2. Harris, 1956; 3. Pace and Elrod, 1960;
4. Packer, Papa, Rust and Ajl, 1959; 5. Pomerat and Willmer, 1939; 6. Siegel
and Cailleau, 1956.
that respiration could fluctuate widely, depending both on the conditions of culture and the conditions under which respiration was
measured. It should therefore be noted that they may not represent
maximum values.
Most authors have reported very low values for endogenous carbohydrate in cultured cells (Gill, 1938; Nirenberg, 1958; Peppers,
Westfall and Earle, 1959; Roussy and Craciun, 1928) but W u (1959)
falls within the above range and although each cell has its own
characteristic value this does not seem to be closely correlated with its
glycolytic capacity. On the whole it seems to be more closely related
to the amount of cytoplasm in the cell (Phillips et al., 1960). Most ofthe
results reported in Table I V were reported before it was appreciated
T A B L E V
Effect o f various substances o n growth and metabolism