246
J O H N
P A U L
Table III cont.
Carbohydrate
g
§
•I 1 .3
o HS ffi
W3
cell
Chick hearl
fibroblast
References
KB
L
Hum
fibrol
Liver
MBU
Chick hearl
fibroblast
Cryst
lens
Polysaccharides
Dextrin
Glycogen*
Insulin
Starch
+
0 0 / + +
0
0
0
Phosphorylated
compounds*
Glucose-l-P0 4
-
- + + + + - + + -
- + +
Glucose-6-P0 4
-
- + + + + - + + -
- + +
Fructose-6-P0 4
-
-
-
-
-
-
-
- 0
Fructose-1-6-P0 4
-
-
-
-
-
-
-
- 0
Ribose-5-P0 4
_
_
_
_
_
_
_
-
0
Na glycerophosphate -
-
-
-
-
-
-
- 0
Na phosphoglycerate -
-
0
0
-
-
-
-
0
4, 3 and 2-carbon
compounds
Pyruvate*
0
0
0
0
0
0
0
-
0
Citrate
_
_
0
-
0
-
0
0
0
Succinate
_
_
0
-
0
-
0
-
0
Fumarate
_
_
0
-
0
-
0
-
0
Malate
_
_
0
-
0
-
0
-
0
Lactate*
_
_
0
-
0
-
-
+
0
D-erythrose
-
-
0
-
0
-
-
-
-
Acetate
_
_
0
-
0
-
0
0
-
Glycerol
_
_
0
-
0
-
0
+
0
Hexonic and
Hexuronic acids
Glucuronic acid
-
-
-
0 -
-
0
0
0
Galacturonic acid
-
-
-
0 -
-
0
-
0
Phosphogluconic acid
-
-
-
-
-
-
-
-
0
Gluconic acid
-
-
-
0
-
-
0
-
-
2
2, 3,4
2
2 , 4
2,3
2,3
2
2
2
2
1,2
1,2
1,2,4
1.2
1,2
1,2
1,2,4
1,3
1,4
1,2,4
1,2,4
1,2
2
1
+ + , as effective as D-glucose; + , distinctly less effective but will permit survival and
slow growth; 0, completely ineffective; - , not tested.
*See text for qualifications.
References: 1. Eagle, Barban, Levy and Schulze (1958); 2. Morgan and Morton (1960);
3. Harris and Kutsky (1953); 4. Bailey, Gey and Gey (1959); 5. Wachtl and
Kinsey (1957).
mannose and trehalose can substitute for glucose. There seems to be
some variation in the capacity of different cell strains to utilize pentoses
and other sugars. It should be noted that not only were three different
kinds of culture material investigated in these studies (established mammalian cell strains, freshly isolated mammalian lens and freshly isolated
J O H N
P A U L
Table III cont.
Carbohydrate
g
§
•I 1 .3
o HS ffi
W3
cell
Chick hearl
fibroblast
References
KB
L
Hum
fibrol
Liver
MBU
Chick hearl
fibroblast
Cryst
lens
Polysaccharides
Dextrin
Glycogen*
Insulin
Starch
+
0 0 / + +
0
0
0
Phosphorylated
compounds*
Glucose-l-P0 4
-
- + + + + - + + -
- + +
Glucose-6-P0 4
-
- + + + + - + + -
- + +
Fructose-6-P0 4
-
-
-
-
-
-
-
- 0
Fructose-1-6-P0 4
-
-
-
-
-
-
-
- 0
Ribose-5-P0 4
_
_
_
_
_
_
_
-
0
Na glycerophosphate -
-
-
-
-
-
-
- 0
Na phosphoglycerate -
-
0
0
-
-
-
-
0
4, 3 and 2-carbon
compounds
Pyruvate*
0
0
0
0
0
0
0
-
0
Citrate
_
_
0
-
0
-
0
0
0
Succinate
_
_
0
-
0
-
0
-
0
Fumarate
_
_
0
-
0
-
0
-
0
Malate
_
_
0
-
0
-
0
-
0
Lactate*
_
_
0
-
0
-
-
+
0
D-erythrose
-
-
0
-
0
-
-
-
-
Acetate
_
_
0
-
0
-
0
0
-
Glycerol
_
_
0
-
0
-
0
+
0
Hexonic and
Hexuronic acids
Glucuronic acid
-
-
-
0 -
-
0
0
0
Galacturonic acid
-
-
-
0 -
-
0
-
0
Phosphogluconic acid
-
-
-
-
-
-
-
-
0
Gluconic acid
-
-
-
0
-
-
0
-
-
2
2, 3,4
2
2 , 4
2,3
2,3
2
2
2
2
1,2
1,2
1,2,4
1.2
1,2
1,2
1,2,4
1,3
1,4
1,2,4
1,2,4
1,2
2
1
+ + , as effective as D-glucose; + , distinctly less effective but will permit survival and
slow growth; 0, completely ineffective; - , not tested.
*See text for qualifications.
References: 1. Eagle, Barban, Levy and Schulze (1958); 2. Morgan and Morton (1960);
3. Harris and Kutsky (1953); 4. Bailey, Gey and Gey (1959); 5. Wachtl and
Kinsey (1957).
mannose and trehalose can substitute for glucose. There seems to be
some variation in the capacity of different cell strains to utilize pentoses
and other sugars. It should be noted that not only were three different
kinds of culture material investigated in these studies (established mammalian cell strains, freshly isolated mammalian lens and freshly isolated