11. SEROTYPING OF LZSTERIA MONOCYTOGENES AND RELATED SPECIES 43
T A B L E I V
Scheme for preparation- of 0-factor sera for Listeria tnonocytogenes
Listeria monocytogenes
0-factor
rabbit 0-antiserum
1
1/11
IV
V/VI
VI
VII
VIII
I X
X
X I
XII/XIII
XI11
XIV
XV
1
1 part 112 a mixed with
1 part 1/2 b
3
4b
4b
4c
4d
4a
5
6
7
7
IM. grayi
4f
Absorbed with
0-antigens
from serotype
3
4
1
1
4c
4b
4b
4c
4d
4c + 4d
M . grayi
7
4b
T A B L E V
Absorption procedure for Listeria H-factor sera
Listeria monocytogenes
rabbit OH-antiserum
H-factors
from serotype
A
1 /2a
AB
1 /2a
C
4b
D
1 /2c
Absorbed with
0-antigens
from serotype
1 /2a
1 /2a
4b
1 /2c
Absorbed with
OH-antigens
from serotype
1 /2c
1 /2a
1 /2a
-
For the preparation of OH-factor sera, two controls are necessary.
1. Control with 0-antigen used for absorption: no agglutination allowed.
2. Control with OH-antigen of serotype 1/2a, 1/2c and 1/2b, agglutination as
follows :
OH-antigen
OH-antigen
OH-antigen
from
from
from
H-factors
serotype 1 /2a
serotype 1/2c
serotype 1/2b
-
A
+
0
+
AB
+
+
+
C
0
0
+
D
0
+
0
~~
~~
~
~~
~
~~
~~
If the reaction is significantly positive, serum has to he diluted with two parts
of 0.85% saline containing 0.5% phenol.