42
H. P. R. SEELIGER AND K. HOHNE
Pure H-antisera are produced by absorption of OH-antisera with
homologous 0-antigen.
Kunkel and Naumann reported in 1974 on the preparation of a pure
H-antigen by adsorbing Listeriu flagella to latex particles. Their efforts
did not result in an agglutination between the flagella-latex-mixture and
the H-antisera.
D. Production of factor sera
Factor sera may be produced with strains selected and recommended by
Seeliger and Donker-Voet (see Table 111) which are available from the
National Collection of Type Cultures London, The ATCC, Rockville,
Md., US4, and the Listeria Culture Collection of the Institute for Hygiene
and Microbiology, Wiirzburg, FRG. The procedure for obtaining factor
sera by CASTELLANI’s absorption test is outlined on Table V.
T A B L E I11
List of strains used for serological characterisation of Listeria rnonocytogenes and related species
Collection No Wiirzburg
Serotype
SLCC
Also listed
_ . ~
1 /2a
2371
NCTC 7973/ATCC 191 11
1 /2c
2372
NCTC 5348/ATCC 191 12
3a
2373
NCTC 5105/ATCC 19113
1 /2b
2755
DONKER-VOET 1684
3b
2540
DONKER-VOET L471
3c
2479
DONKER-VOET 1916
4a
2374
NCTC 5214/ATCC 19114
4b
2375
ATCC 19115
4c
2376
ATCC 19116
4d
2377
ATCC 19117
4e
2378
ATCC 19118
4f
3379
4g
3423
5
2379
ATCC 19119
7
2482
DONKER-VOET 1627
M. grayi
2861
ATCC 19120
Listeria denitrificans
2836
Collection Institut Pasteur Paris
6 (sub judice)
2480
DONKER-VOET 1383
SLCC : Special Listeria Culture Collection, Wurzburg, F.R.G.
NCTC: National Collection of Type Cultures, London, G.R.
DONKER-VOET: C/O Listeria Collection, Rijks Instituut voor de Volksgezondheid, Biltoven, Netherlands.