VIII. METHODS FOR TYPING MYCOBACTERIA
361
and host strain nomenclature and the recognised phage types are shown in
Tables I1 and 111. When these methods are followed meticulously, the data
obtained are highly reproducible among different laboratories.
Subsequent studies have now appeared in which were used the
methodology and the typing phages recommended by the World Health
T A B L E I 1
Mycobacteriophages used €or phage typing Mycobacterium
tuberculosis
Present Original designation
designation
and 1 x RTDa
Host
Source
MTPHb1 A G l
1 x 105 AT7
W. B. Redmond,
M T P H 2 DS6A
1 x 106 M . tuberculosis, H37Rv
W . B. Redmond,
M T P H 3 GS4E
1 x 105 M . tuberculosis, H37Rv
W . B. Redmond,
M T P H 4 BK1
1 x 106 M. smegmatis, ATCC 607 I . Baess,
~~
~~~
~
M . kansasii
Atlanta, U.S.A.
Atlanta, U.S.A.
Atlanta, U.S.A.
Copenhagen, Denmark
M T P H S BGl
4 x 103 M . intracellularec P-17
W . B. Redmond,
lLlTPH6 D-34
1 x lo7 Mycobacterium not
S. Froman,
MTPH 7 DNA 111 8 1 x 105 M . tuberculosis, H37Rv
E. Mankiewicz,
M T P H 8 X-20
1 x 106 M . tuberculosis, H37Rv
J. H. Bates,
M T P H 9 PH
1 x 105 M . tuberculosis, H37Rv
K. Sushida,
M T P H 10 Clark
1 x 106 M . snzegmatis, ATCC 607 E. Mankiewicz,
MTPH 11 Sedge
1 x 106 M . smegmatis, ATCC 607 E. Mankiewicz,.
MTPH 12 Legendre
1 x 106 M . smegmatis, ATCC 607 E. Mankiewicz,
(Run yon)
Atlanta, U.S.A.
speciated, Froman’s F-130 Los Angeles, U.S.A.
Montreal, Canada
Little Rock, U.S.A.
Tokyo, Japan
Montreal, Canada
Montreal, Canada
Montreal, Canada
a Minimal concentration of virus (PFU/ml) that gave almost confluent lysis
when 0.01 ml of stock was “spotted” on propagating host strain. RTD=routine
test dilution.
M T P H = mycobacterial typing phage, human.
c This virus was titrated on ATCC 607.
This Table is reproduced by permission from the American Review of Respiratory Diseases 111, 460.