IV. INVESTIGATION OF C‘. DIPHTHERIAE
163
heart infusion agar (BHIA) and brain--heart infusion broth (BHIB) (Saragea
et pl., 1972b and unpublished data; Toshach et al., 1977).
For typing procedures the solid medium recommended is BHIA Difco
with a concentration of agar 1.1%.
T o each 100 ml of 1.1% agar (BHIA) are added aseptically:
“Thiamine HC1 O.Olyo
0.1 ml
D-L-Tryptophane 0.1%
1 ml
tL-Cystine 1 yo
0.1 ml
CaC12, 1 M
0.1 ml
Glucose 10%
2 ml
Bovine or horse serum
10 ml
Final concentration of agar
0.97%
For fresh cultures, phage stock preparation and lysogenicity, BHIB
Techniques for performing phage typing, bacteriocin typing and lysoDifco medium supplemented with 0.001 % L-cystine is recommended.
genicity were described in the previous Sections.
XIV. PRINCIPLES O F EPIDEMIOLOGICAL METHODOLOGY
A. General
Dubos remarked in 1952, “medical microbiology is the study of hostparasite relationships and not that of micro-organisms alone, considered
as independent living agents”. This principle may be further extended to
community-parasite relationships, i.e. the epidemiology of the organisms.
Accordingly the general principles recommended for the public health
fight against diphtheria will be mentioned.
These principles are complex, as is evidenced by extensive practical and
theoretical research of the authors of this Chapter in Romania in a vast
field experiment, lasting about 20 years and extending throughout the
country.
The epidemiological features of diphtheria, as well as socioeconomic
factors, determine the strategy and tactics of public health services in the
prevention and control of this disease. This activity involves a scientific
programme of surveillance and control.
In Romania, in 1955, in spite of compulsory diphtherial immunisation,
* Amino acid solutions are filtered through Millipore membranes.
t L-Cystine is prepared as described in the previous Section.
I N T H E PREVENTION AND CONTROL O F DIPHTHERIA
163
heart infusion agar (BHIA) and brain--heart infusion broth (BHIB) (Saragea
et pl., 1972b and unpublished data; Toshach et al., 1977).
For typing procedures the solid medium recommended is BHIA Difco
with a concentration of agar 1.1%.
T o each 100 ml of 1.1% agar (BHIA) are added aseptically:
“Thiamine HC1 O.Olyo
0.1 ml
D-L-Tryptophane 0.1%
1 ml
tL-Cystine 1 yo
0.1 ml
CaC12, 1 M
0.1 ml
Glucose 10%
2 ml
Bovine or horse serum
10 ml
Final concentration of agar
0.97%
For fresh cultures, phage stock preparation and lysogenicity, BHIB
Techniques for performing phage typing, bacteriocin typing and lysoDifco medium supplemented with 0.001 % L-cystine is recommended.
genicity were described in the previous Sections.
XIV. PRINCIPLES O F EPIDEMIOLOGICAL METHODOLOGY
A. General
Dubos remarked in 1952, “medical microbiology is the study of hostparasite relationships and not that of micro-organisms alone, considered
as independent living agents”. This principle may be further extended to
community-parasite relationships, i.e. the epidemiology of the organisms.
Accordingly the general principles recommended for the public health
fight against diphtheria will be mentioned.
These principles are complex, as is evidenced by extensive practical and
theoretical research of the authors of this Chapter in Romania in a vast
field experiment, lasting about 20 years and extending throughout the
country.
The epidemiological features of diphtheria, as well as socioeconomic
factors, determine the strategy and tactics of public health services in the
prevention and control of this disease. This activity involves a scientific
programme of surveillance and control.
In Romania, in 1955, in spite of compulsory diphtherial immunisation,
* Amino acid solutions are filtered through Millipore membranes.
t L-Cystine is prepared as described in the previous Section.
I N T H E PREVENTION AND CONTROL O F DIPHTHERIA