142
FREEZE—DRYING
.
M Morphology of Pancreas and Liver npregnated With Protective Agents
.
,
Pancreatic cells immersed for 5 minutes in DMSO (20 %) and then frozen and sUbstitùted
can be seen in Figures 10 and 11. Note the
structure in the ER, Figure'lO,
.
ribosomes, and mitochondrialrembranæ (see below for details) Figure 11. It must be
emphaSized that this is not always the result obtained, in that some areas of the tissue still
.
possess ice crystals and some other regions, f0rms of damage most likely dueto effects of
the DMSO.
'
‘
‘
Liver tissue impregnated with 20 %—25 % glycerol in Kreb’s Ringer solution for 15 minutes
‘
at room temperature may be seen in Figure 12. Cytoplasmic areas appear to be divided into
those containing glycogen (by inference from chemically xed material) and those cotai—
ning mitochondria,_ER, Golgi, etc., Figures 12 and 13. Mitochondrial cristae generally
appear as spaces about ZOO—230 Àwide traVersed by a line which bisects this space, Figure 14.
(This micrograph is, of glyœrol _impregnated pancreas rather than liver, since pancreatic
,ÏÏ
mitochondria have more and more regularly arranged cristae and show the double unit
”
ê
membrane» structure in
sections.» The results are similar but less favorably seen in
liver.) The
possesses this structure with the same dimensions, Figure 15.
The matrix is extremely dense after staining With_uranium, lead or permanganate, and the
outer dense lines in the cristaearediîcult toSee. However, in DMSO, as seen above, the
matrix does
as dehsely and
seen that the cristae consistof three dense
lines
by 50—60 Â spaces as the other mitochondrial membranes do in both types
ofpreparation, In some parts of tissue the criStae appear as irregular spaces up
400—500 Â
_
wide. These are places
Considerable tissue damage by Other criteria,
such as extreme irregularity of the ER, strigineSs ofthe cÿtoplasm, etc. The interpretation
'
which seems to t our observations
the outer mitochondrial membrane
the cristae consist of unit membranes and_that all space has been eliminated between outer
"
and inner mitochondrial membrane and'the two membranes of the cristae. This leaves the
‘iimer’ components of the unit membranes inintimate contact. Whether
is
normal
condition 0]: that with the space between membranes is normal is, of course, not known.
The ER again, consists ”of unitmembranes With ribo‘somes attached. GOlgi regions are
‘tight’ and consist of layers of unit membranes With elongated vacuoles, partly lled with
'
material which appears to be excluded from spherical 400 À spaces, Figure 16. The vacuoles
appear to be primarily On one face of the Golgi‘regios, Figure 16.
stain remarkablÿ densely,
and
Note the chro'matin regions,
nuclear pore rcägions,‘ucledli “(identied primarin on a m0rph010gicalbaSis) and espeCially
thewealth» Of—intertähromatinic material.“
space
the nucleus is 'ühdoubædly due
'
to shrihkage; In favorable "sections, one‘îmay'see each margin of the space
by that
appears to be.a unit:membranezaNo dierentiatedæim has as yet“ been seen ass00iaæd With
Bundles=of
invarious parts ofthese ‘sectionsad
a-considerable“;number
-
…
:.
>
*
:
_
,_
-
f--ï:
‘ -
î
our-use
..onimaræine‘eggain
_
preparation: £or; rapid;œezig, &…:an «rpary; because?
31h&YÔlfb6©-Ææd by ;…us 1n
is@1atingüemt0üc apparatus
-
gated the 'e‘eCts of these agents on
intact eggin; cell—division: TO
We
FREEZE—DRYING
.
M Morphology of Pancreas and Liver npregnated With Protective Agents
.
,
Pancreatic cells immersed for 5 minutes in DMSO (20 %) and then frozen and sUbstitùted
can be seen in Figures 10 and 11. Note the
structure in the ER, Figure'lO,
.
ribosomes, and mitochondrialrembranæ (see below for details) Figure 11. It must be
emphaSized that this is not always the result obtained, in that some areas of the tissue still
.
possess ice crystals and some other regions, f0rms of damage most likely dueto effects of
the DMSO.
'
‘
‘
Liver tissue impregnated with 20 %—25 % glycerol in Kreb’s Ringer solution for 15 minutes
‘
at room temperature may be seen in Figure 12. Cytoplasmic areas appear to be divided into
those containing glycogen (by inference from chemically xed material) and those cotai—
ning mitochondria,_ER, Golgi, etc., Figures 12 and 13. Mitochondrial cristae generally
appear as spaces about ZOO—230 Àwide traVersed by a line which bisects this space, Figure 14.
(This micrograph is, of glyœrol _impregnated pancreas rather than liver, since pancreatic
,ÏÏ
mitochondria have more and more regularly arranged cristae and show the double unit
”
ê
membrane» structure in
sections.» The results are similar but less favorably seen in
liver.) The
possesses this structure with the same dimensions, Figure 15.
The matrix is extremely dense after staining With_uranium, lead or permanganate, and the
outer dense lines in the cristaearediîcult toSee. However, in DMSO, as seen above, the
matrix does
as dehsely and
seen that the cristae consistof three dense
lines
by 50—60 Â spaces as the other mitochondrial membranes do in both types
ofpreparation, In some parts of tissue the criStae appear as irregular spaces up
400—500 Â
_
wide. These are places
Considerable tissue damage by Other criteria,
such as extreme irregularity of the ER, strigineSs ofthe cÿtoplasm, etc. The interpretation
'
which seems to t our observations
the outer mitochondrial membrane
the cristae consist of unit membranes and_that all space has been eliminated between outer
"
and inner mitochondrial membrane and'the two membranes of the cristae. This leaves the
‘iimer’ components of the unit membranes inintimate contact. Whether
is
normal
condition 0]: that with the space between membranes is normal is, of course, not known.
The ER again, consists ”of unitmembranes With ribo‘somes attached. GOlgi regions are
‘tight’ and consist of layers of unit membranes With elongated vacuoles, partly lled with
'
material which appears to be excluded from spherical 400 À spaces, Figure 16. The vacuoles
appear to be primarily On one face of the Golgi‘regios, Figure 16.
stain remarkablÿ densely,
and
Note the chro'matin regions,
nuclear pore rcägions,‘ucledli “(identied primarin on a m0rph010gicalbaSis) and espeCially
thewealth» Of—intertähromatinic material.“
space
the nucleus is 'ühdoubædly due
'
to shrihkage; In favorable "sections, one‘îmay'see each margin of the space
by that
appears to be.a unit:membranezaNo dierentiatedæim has as yet“ been seen ass00iaæd With
Bundles=of
invarious parts ofthese ‘sectionsad
a-considerable“;number
-
…
:.
>
*
:
_
,_
-
f--ï:
‘ -
î
our-use
..onimaræine‘eggain
_
preparation: £or; rapid;œezig, &…:an «rpary; because?
31h&YÔlfb6©-Ææd by ;…us 1n
is@1atingüemt0üc apparatus
-
gated the 'e‘eCts of these agents on
intact eggin; cell—division: TO
We