’
_
\
.
:
3
«L
+
t
_
FIGURE ]. Rat pancreas rapidly frozen by formwar sandwich technique. N : nucleus. Arrow points to a
mitochondrion. Endoplasmic reticulum can be seen in the cytoplasm, but is distorted by “reticulum ”we
are assuming due to ice crystallization. >< 6,400.
FIGURE 2. Spisula oocyte dehydrated in 100—30 sea water and rapidly frozen after sandwiching. V = vitelline
membrane. Proles of parallel array of ER in the cytoplasm. >< 8,000.
FIGURE 3. Cooling curves obtained by method outlined in Rebhun 1965. Top horizontal trace is 0 °C, lower
horizontal trace —— 197 0C. Curve l. Cooling curve of thermocouple alone. Break in the curve at the number 1 marks inception of nucleate boiling. Curve 2. Thermocouple coated with dibutylphtalate. Boiling region
occurs sooner and results in overall decrease in freezing rate but no initial decrease. Curve 3. Thermocouple
\
coated with DBPh and then With Santocel powder. Boiling begins immediately.
_
FIGURE 4. Mitochondrion from Spisula egg osmotically dehydrated in 100—67 sea water (Le. — 3 x sea water).
Note unit membranes “back to back” in cristae. >< 80,000.
_
\
.
:
3
«L
+
t
_
FIGURE ]. Rat pancreas rapidly frozen by formwar sandwich technique. N : nucleus. Arrow points to a
mitochondrion. Endoplasmic reticulum can be seen in the cytoplasm, but is distorted by “reticulum ”we
are assuming due to ice crystallization. >< 6,400.
FIGURE 2. Spisula oocyte dehydrated in 100—30 sea water and rapidly frozen after sandwiching. V = vitelline
membrane. Proles of parallel array of ER in the cytoplasm. >< 8,000.
FIGURE 3. Cooling curves obtained by method outlined in Rebhun 1965. Top horizontal trace is 0 °C, lower
horizontal trace —— 197 0C. Curve l. Cooling curve of thermocouple alone. Break in the curve at the number 1 marks inception of nucleate boiling. Curve 2. Thermocouple coated with dibutylphtalate. Boiling region
occurs sooner and results in overall decrease in freezing rate but no initial decrease. Curve 3. Thermocouple
\
coated with DBPh and then With Santocel powder. Boiling begins immediately.
_
FIGURE 4. Mitochondrion from Spisula egg osmotically dehydrated in 100—67 sea water (Le. — 3 x sea water).
Note unit membranes “back to back” in cristae. >< 80,000.