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FIGURES 12 and 13. General view of cytoplasm and nuclei in rat liver impregnated with 20 % glycerol prior to
freezing. Note mitochondria with “negative” cristae, microbodies (arrow). ER, and nuclear pore regions
(with shrinkage space around nucleus). Relatively empty areas are presumed to have been occupied by
glycogen in life. This implication derives from comparison with glutaraldehyde xed cells.
Figure 12 : >< 16,000.
Figure 13 : >< 12,000.