.
Details of procedure for predicting titer
‘
Experimental Data2
Standard Deviation of Rate
Temperature
(Zty _ __2t
Ëy)2
‘
°
2
(Ëy)2
n
,
!‘
28 °C
36.2 °C
45
C
Ëy
<
‘
t
Y
t
Y
t
Y
S°D°"1 :
,
,.
time
titer
time
titer
time
titer
___—__—
;
.
days
lag
days
lag
days
log
(n __ 2)
[Et2 _ Œt)2]
0.0
4.851
0.0
4.851
0.0
4.851
…
”
_,
0.5
4.76
0.5
4.76
0.5
3.58
_
1
4.52
—
1
4.45
1
2.86
t
d d E
R t
2
4.34
2
3.70
2
2.13
S ”" ‘”
”°” of a e
3
4.27
3
3.24
3
2.05
S-E-k1 :
_S—D_JcL
.
4
3.94
4
2.67
4
1.72
_
.
/n _. 1
'
_5
3.78
5 ,
2.61
6 ,
3 .48
6
2.30
,
7
345
7
238
Statistical Values
8
3.11
'
«
8
2.23
Statistic
.
Temperature
10
2.92
10
1.32
28 oc
36.2 oc
45 oc
12
3.07
_
15
252
.
.
k1
-——0.115
—0.326
—
0.484
Arithmetic Calculatz‘ons
Standard Error of D erence
,.
14
_
10
5
'
,
S.E.… = JS.E.;. + S.E.f…
2:
118.5
46.5
10.5
:
k:
SPE—au
.
Ët2
1,698.25
304.25
30.25
.,
°
'
(Et)2
14,04225
‘
«
2,162.25
110.25
âä €
ÎË'2 %
g-gëä
$;»
48.31
29.06
12.34
36 2 ..C
45
°c
0'057
Ey2
176.78“
98.25
_
32.69
'
'
_
-
(2y)2
2,333.85 ; 4
876.71
152.27
,
2 (ty)
328.55
109.18
21.94
Slope Di”erenœs in Terms of Standard Errars '
‘
k1 — k:
_
Rate of Degradation
_
t =
S
,
Et 2
,
'
2
ki
k;
t** 8
,
.
k1 =
“““—(Éty—
,
28
°C
36.2 °C
_
17.22
'
Et2—=Ï
'
-
28
°C
45
°C
6.53
“
_
"
__
,,
°C -
45
2.74
‘
,
’
'—
1 Repril1_ted_ from the ,.Îournaliôfä1m1ùolôgy: by permiss‘ion_ôf Williams and Wilkis, Publishers.
the tit0rs at zer0 time“ are 110tÇ_'VälûÔSÏÎÔI deg1“adation of virus; they were not used for statistical
..Çî:I;-‘3ïï
,
—
_
;‘afrcalculaüons4
,
ff
-‘_
…
—
‘
%
of t— equal to or.greater
that the difference; obtained are statistically signi'
Details of procedure for predicting titer
‘
Experimental Data2
Standard Deviation of Rate
Temperature
(Zty _ __2t
Ëy)2
‘
°
2
(Ëy)2
n
,
!‘
28 °C
36.2 °C
45
C
Ëy
<
‘
t
Y
t
Y
t
Y
S°D°"1 :
,
,.
time
titer
time
titer
time
titer
___—__—
;
.
days
lag
days
lag
days
log
(n __ 2)
[Et2 _ Œt)2]
0.0
4.851
0.0
4.851
0.0
4.851
…
”
_,
0.5
4.76
0.5
4.76
0.5
3.58
_
1
4.52
—
1
4.45
1
2.86
t
d d E
R t
2
4.34
2
3.70
2
2.13
S ”" ‘”
”°” of a e
3
4.27
3
3.24
3
2.05
S-E-k1 :
_S—D_JcL
.
4
3.94
4
2.67
4
1.72
_
.
/n _. 1
'
_5
3.78
5 ,
2.61
6 ,
3 .48
6
2.30
,
7
345
7
238
Statistical Values
8
3.11
'
«
8
2.23
Statistic
.
Temperature
10
2.92
10
1.32
28 oc
36.2 oc
45 oc
12
3.07
_
15
252
.
.
k1
-——0.115
—0.326
—
0.484
Arithmetic Calculatz‘ons
Standard Error of D erence
,.
14
_
10
5
'
,
S.E.… = JS.E.;. + S.E.f…
2:
118.5
46.5
10.5
:
k:
SPE—au
.
Ët2
1,698.25
304.25
30.25
.,
°
'
(Et)2
14,04225
‘
«
2,162.25
110.25
âä €
ÎË'2 %
g-gëä
$;»
48.31
29.06
12.34
36 2 ..C
45
°c
0'057
Ey2
176.78“
98.25
_
32.69
'
'
_
-
(2y)2
2,333.85 ; 4
876.71
152.27
,
2 (ty)
328.55
109.18
21.94
Slope Di”erenœs in Terms of Standard Errars '
‘
k1 — k:
_
Rate of Degradation
_
t =
S
,
Et 2
,
'
2
ki
k;
t** 8
,
.
k1 =
“““—(Éty—
,
28
°C
36.2 °C
_
17.22
'
Et2—=Ï
'
-
28
°C
45
°C
6.53
“
_
"
__
,,
°C -
45
2.74
‘
,
’
'—
1 Repril1_ted_ from the ,.Îournaliôfä1m1ùolôgy: by permiss‘ion_ôf Williams and Wilkis, Publishers.
the tit0rs at zer0 time“ are 110tÇ_'VälûÔSÏÎÔI deg1“adation of virus; they were not used for statistical
..Çî:I;-‘3ïï
,
—
_
;‘afrcalculaüons4
,
ff
-‘_
…
—
‘
%
of t— equal to or.greater
that the difference; obtained are statistically signi'