.
'
—
108c
APPENDIX A
Freezing, cyclic freezing and thawing, low temperature storage andfreeZe—dryirzg
DATE OF EXPERINŒNT
:
.
’
BASIC SÙSPENDING MEDIUM (BSM) :
_
—
PREPARATION OF VIRUS LOT USED :
'
DATE
'
'
,'
HOST FÜR GROWTH
METHOD
FOR PURŒICATION, CLARIEICATION, CONCENTRATION :
EXPRESSION OF ACTIVITY
:
'
DŒEERENCE
FOR SIGNŒICANCE :
LABORATORY
CHARACTERISTICS OF VIRUS (encircle) :
,
‘
:
'
RNA - DNA;
Solvant Sensitive - SOlvent
Envelope“ - Naked;
'
pH labe - pH stable;
-
Heatstable
'
VIRUS
-' '
'
‘
CODE PREle (Date)
-
AMOUNT OF SUSPENSION/ TUBE :”-----—'---------- —-—---—--—--——-----—-—-----—-------—-----—--“""'““""'"'°""""""'""“""“"""'
_
CODE
DILUENT
TELŒERATÜREANDRÀTE(C)ACHVITŸ
_
:
A—1
CONTROL
=
‘
f*Tÿ‘
‘
"
A—2
CONTROL
_
a
B—1
BSM
—40810Wy
'
fÏ
f
'
-
B—2
BSM .
.
—40°,Sk)Wly
_
C—l
BSM + PDA
"
:
D—1
-
BSM +…2 % DMSOf1
;
—4üslowlY
=
D-2 ,
BSM +52 %
»
…
,
—40°,810W1Y
"
’
"
'
'
l
-
El
;BSM
—-76slowly
_=*»