'
'Ïhe
the Tpreffæzingçsrage»
,
.
'
'
the Vi&hity
,
of
organiSm‘s ' _
'
=
'
'
_
;
mousson or
on
.
,
.
,
,
:
Although it is over 50 years
drying, no success has
in pre's‘erving more compleX‘ nucleated living cells let
alone more complex mültiCellùlat creatures.
‘
Î
"
The initial successes in “préserving bacteria by
were
purély
empirically, but it required müCh"reseafch_ over many" years into the‘media m Wh10hthé
organisms were suspended,‘ which‘proved
be the mostimpor‘tant single
ing high survival rates. Even today, Without increased koWledge "Ofth‘e'
.
tituents of a drying
there are
Which survivë‘the
poov
Ç
,
Ï
.
[_
Freezing, thawing and
fall
Which are potentiällÿ ‘lethal‘to hvmgcells
At one time it was
sincefreeZË—difÿing avoi@ed the thaWing‘ process,this7technique
-
might be less lethal than
butsofaf,hä%
has .il—”Qt Pr°î‘îê”di9
It is essential if … Pî931“639 lstobemade 1nîh18eïdtha’“ thefreezethaWïlmy Sh°“1d
be differentiaæd
“Cheldïyîäml‘lïy 0ntheomhandVCh0kmeseem“°bevefy
resistant to freeze—thäw 1murybutverysuscepüblet0drymgmary Ontheothefhand
_
there are a number of organismsWhich are relat1velyverysusœp*üble tofreezmgmmry
and, at the moment, in. our laboratory, We are concentrating‘ On the freezç;drymg Of these.
it was Luyet (1951) Who ï”Sf
'
…
Cells … d»bymefmtfynHdd
'
.
enough there
'»