80
chI a that the Maize plants had - exactly as is the case with oceanic Prochlorophytes, which
do not contain a trace of normal chI a (Chisholm et al., 1988; surprisingly enough, freshwater
Prochlorophytes and Prochloron sp. do not contain divinyl-Chl a: Burger-Wiersma et al.,
1986; Foss et at., 1987). There are other reports of algae photosynthesizing in spite of a lack
of normal chI a (Kowalewska et al., 1987; Kowalewska, 1988).
4
12hrs.
at 50;:; light
3
Figure 12. Results of transfer experiments with Sargasso Sea samples containing the red-shifted "divinyl-Chl a":
photobleaching. Fluorescence scans of thin-layer chromatograms developed in one direction. Peak 2:
Divinyl-Chl b. Peak 3: Divinyl-Chl a.
Our own attempts to characterize the marine Prochlorophyte's chI a and b by NMR, including
COSY, have not been successful due to difficulties of sampling enough material (Van Dijken,
1990). We intend to use Fast Atom Bombardment mass spectroscopy and Time-of-Flight mass
spectroscopy (as developed by 1. Grotemeyer, Germany) for further characterization of the
red-shifted chlorophylls a and b of oceanic picoplanktonic Prochlorophytes, in cooperation
with Dr. W. Wierenga, Dept. of Chemistry, Groningen University, and Prof. 1.1. Boon,
F.O.M. Institute, Amsterdam.
chI a that the Maize plants had - exactly as is the case with oceanic Prochlorophytes, which
do not contain a trace of normal chI a (Chisholm et al., 1988; surprisingly enough, freshwater
Prochlorophytes and Prochloron sp. do not contain divinyl-Chl a: Burger-Wiersma et al.,
1986; Foss et at., 1987). There are other reports of algae photosynthesizing in spite of a lack
of normal chI a (Kowalewska et al., 1987; Kowalewska, 1988).
4
12hrs.
at 50;:; light
3
Figure 12. Results of transfer experiments with Sargasso Sea samples containing the red-shifted "divinyl-Chl a":
photobleaching. Fluorescence scans of thin-layer chromatograms developed in one direction. Peak 2:
Divinyl-Chl b. Peak 3: Divinyl-Chl a.
Our own attempts to characterize the marine Prochlorophyte's chI a and b by NMR, including
COSY, have not been successful due to difficulties of sampling enough material (Van Dijken,
1990). We intend to use Fast Atom Bombardment mass spectroscopy and Time-of-Flight mass
spectroscopy (as developed by 1. Grotemeyer, Germany) for further characterization of the
red-shifted chlorophylls a and b of oceanic picoplanktonic Prochlorophytes, in cooperation
with Dr. W. Wierenga, Dept. of Chemistry, Groningen University, and Prof. 1.1. Boon,
F.O.M. Institute, Amsterdam.