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Figure 2. Phylogenetic identification of single microbial cells using ill situ hybridization with fluorescently
labeled oligonucleotide probes.A mixed culture of Saccharomyces and E. coli hybridized with a
rhodamine-labeled eukaryotic probe and a fluorescein-labeled prokaryotic probe and counterstained with
(Hoechst) BBI-33342 DNA dye at I jLM. Images acquired with a Photometries 2100 cooled CCD
(Kodak 1035 x 1280 pixels) camera interfaced to a MacIntosh Cfx computer, and processed by
TCL-image and NIH-image software. A. phase contrast; B rhodamine channel: excitation 546 nm,
emission >590 nm; C. fluorescein channel: excitation 440-480 nm, emission 510-550 nm; D.BBI
channel: excitation 365 nm, emission> 450 nm.
An overriding concern to the future of life on our planet is water quality. The composition
as well as the physiological state of marine populations will certainly be some of the criteria
used to measure water quality. Here quantitative cytometric measurements are the key to these
questions. Again, I expect a multi-parameter approach to be the most successful, combining
Figure 2. Phylogenetic identification of single microbial cells using ill situ hybridization with fluorescently
labeled oligonucleotide probes.A mixed culture of Saccharomyces and E. coli hybridized with a
rhodamine-labeled eukaryotic probe and a fluorescein-labeled prokaryotic probe and counterstained with
(Hoechst) BBI-33342 DNA dye at I jLM. Images acquired with a Photometries 2100 cooled CCD
(Kodak 1035 x 1280 pixels) camera interfaced to a MacIntosh Cfx computer, and processed by
TCL-image and NIH-image software. A. phase contrast; B rhodamine channel: excitation 546 nm,
emission >590 nm; C. fluorescein channel: excitation 440-480 nm, emission 510-550 nm; D.BBI
channel: excitation 365 nm, emission> 450 nm.
An overriding concern to the future of life on our planet is water quality. The composition
as well as the physiological state of marine populations will certainly be some of the criteria
used to measure water quality. Here quantitative cytometric measurements are the key to these
questions. Again, I expect a multi-parameter approach to be the most successful, combining