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Figure 1. Confocal immunofluorescence optical sections of a fresh-water protozoa, Euplotes euryslOmus. The
cells were perrneabilized and probed with monoclonal antibodies specific for post-translational modified
carboxy terminal tryosine-tubulin (A) or unmodified carboxy terminal glutamic-tubulin (C). A and C
are CLSM immunofluorescence images; Band C, phase contrast images of the respective animals. The
anti-tyr tubulin is found at the base of the cirri and membranelles whereas the anti-glu tubulin epitope
is found throughout the cirri and membranelles and surrounding the macro- and micro nuclei.
rapidly growing field. In Figure 1 are shown confocal fluorescence images and phase contrast
laser scan images of a fresh-water unicellular Euplores eurystomus stained with monoclonal
antibodies to alpha-tubulin isotypes (Olins et al., 1989). This example demonstrates the
necessity of spatial as well as quantitative fluorescence information for structural and
physiological analysis of such organisms.
Figure 1. Confocal immunofluorescence optical sections of a fresh-water protozoa, Euplotes euryslOmus. The
cells were perrneabilized and probed with monoclonal antibodies specific for post-translational modified
carboxy terminal tryosine-tubulin (A) or unmodified carboxy terminal glutamic-tubulin (C). A and C
are CLSM immunofluorescence images; Band C, phase contrast images of the respective animals. The
anti-tyr tubulin is found at the base of the cirri and membranelles whereas the anti-glu tubulin epitope
is found throughout the cirri and membranelles and surrounding the macro- and micro nuclei.
rapidly growing field. In Figure 1 are shown confocal fluorescence images and phase contrast
laser scan images of a fresh-water unicellular Euplores eurystomus stained with monoclonal
antibodies to alpha-tubulin isotypes (Olins et al., 1989). This example demonstrates the
necessity of spatial as well as quantitative fluorescence information for structural and
physiological analysis of such organisms.