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OTHER APPLICATIONS OF LIGHT SCATTERING IN FLOW CYTOMETRY
E. coli bacteria have been analyzed in a microscope-based flow cytometer using light
scattering and DNA content measurements with the dyes ethidium bromide and Mithramycin
(Boye et al.1983). The light scatter signal was shown to be proportional to bacterial protein
content.
Detailed forward scattering measurements in a flow cytometer were used to distinguish among
four species of microalgae (Price et al. 1978). Scattered light was collected at 32 angles in
the forward direction between 0.2 degrees and 20 degrees. Cluster analysis was used to
distinguish among the complex scattered light patterns from the different microalgae.
Forward and perpendicular light scatter have been used to provide information on the size and
shape of phytoplankton cells (Trask et aI., 1982). The investigators also used intrinsic
chlorophyll fluorescence and the DNA stains Hoechst 33342 and DAPI. These five
measurements on each cell enabled clear distinction among eight algae species.
Forward and perpendicular light scatter in a flow cytometer were used to study intracellular
gas vacuoles in blue-green algae (Dubelaar et al., 1987). The number of gas vacuoles in the
cells was controlled by external pressure changes. The investigators showed that the presence
of gas vacuoles caused a lO-fold increase in perpendicular light scatter and a 5-fold decrease
in forward light scatter.
Spinrad and Brown (1986) used a flow cytometer to measure the relative real refractive index
of a number of marine microorganisms. They measured the light scattered in the forward
direction and near 90 degrees by pure populations of the microorganisms and by calibration
beads with known refractive indices. They used the ratios of the light scattered in the two
directions to determine the refractive indices of the microorganisms. Their measurements
agreed with earlier bulk measurements but showed significant variation among the species of
microorganisms. Ackleson and Spinrad (1988) used a Mie theory model of the scattering
process to estimate the size and refractive index of several nanoplankton species.
OTHER APPLICATIONS OF LIGHT SCATTERING IN FLOW CYTOMETRY
E. coli bacteria have been analyzed in a microscope-based flow cytometer using light
scattering and DNA content measurements with the dyes ethidium bromide and Mithramycin
(Boye et al.1983). The light scatter signal was shown to be proportional to bacterial protein
content.
Detailed forward scattering measurements in a flow cytometer were used to distinguish among
four species of microalgae (Price et al. 1978). Scattered light was collected at 32 angles in
the forward direction between 0.2 degrees and 20 degrees. Cluster analysis was used to
distinguish among the complex scattered light patterns from the different microalgae.
Forward and perpendicular light scatter have been used to provide information on the size and
shape of phytoplankton cells (Trask et aI., 1982). The investigators also used intrinsic
chlorophyll fluorescence and the DNA stains Hoechst 33342 and DAPI. These five
measurements on each cell enabled clear distinction among eight algae species.
Forward and perpendicular light scatter in a flow cytometer were used to study intracellular
gas vacuoles in blue-green algae (Dubelaar et al., 1987). The number of gas vacuoles in the
cells was controlled by external pressure changes. The investigators showed that the presence
of gas vacuoles caused a lO-fold increase in perpendicular light scatter and a 5-fold decrease
in forward light scatter.
Spinrad and Brown (1986) used a flow cytometer to measure the relative real refractive index
of a number of marine microorganisms. They measured the light scattered in the forward
direction and near 90 degrees by pure populations of the microorganisms and by calibration
beads with known refractive indices. They used the ratios of the light scattered in the two
directions to determine the refractive indices of the microorganisms. Their measurements
agreed with earlier bulk measurements but showed significant variation among the species of
microorganisms. Ackleson and Spinrad (1988) used a Mie theory model of the scattering
process to estimate the size and refractive index of several nanoplankton species.