6
Chapter 2 Materials and Methods
Each chapter ends with the bibliography relevant to each taxonomie group and an appendix
with species abundance data for the stations and
depths sampled during the cruise.
2.3 Line Drawings and Scanning
Electron Micrographs
Original ink figures, obtained by use of a camera
lucida, were processed by a computer graphic
system (Apple Maclntosh Quadra with 20 MB
RAM and 128 MB magneto-optic disc). The images were scanned through a UMAX 600 DPI
scanner and then proportionately reduced and
paged through an Al dus Free Hand drawing program.
Specimens for scanning electron microscopy
were prepared according to the method given in
the first volume on copepods. Preserved samples
were washed and left in distilled water for at least
24 h. Amphipods, euphausiids and mysids were
then postfixed in a 2% Os0 4 solution for 1 h prior to dehydration in a graded alcohol series:
50 % for 30 min, 70 % for 30 min, 90 % for
30 min, followed by three washings in 100% alcohol for 30 min each. The procedure was the
same for ostracods except that they were postfixed in a 1% Os0 4 solution and then dehydrated. For chaetognaths, specimens were dehydrated only in the above-mentioned graded alcohol
series. All specimens were then critica! pointdried with liquid C0 2 for 3-5 h. C0 2 was
changed every hour. Specimens were then slowly
brought to a tempera ture of 50 oc and a pressure
of99 atm (1450 psi). The pressure was gradually
brought back to normal within 30 min and specimens were slowly cooled to ambient temperature. The specimens were then mounted on stubs
with adhesive tape and coated with gold for 3
min at a setting of 2.2 k V and 1 O mA of the sputtering apparatus.
Chapter 2 Materials and Methods
Each chapter ends with the bibliography relevant to each taxonomie group and an appendix
with species abundance data for the stations and
depths sampled during the cruise.
2.3 Line Drawings and Scanning
Electron Micrographs
Original ink figures, obtained by use of a camera
lucida, were processed by a computer graphic
system (Apple Maclntosh Quadra with 20 MB
RAM and 128 MB magneto-optic disc). The images were scanned through a UMAX 600 DPI
scanner and then proportionately reduced and
paged through an Al dus Free Hand drawing program.
Specimens for scanning electron microscopy
were prepared according to the method given in
the first volume on copepods. Preserved samples
were washed and left in distilled water for at least
24 h. Amphipods, euphausiids and mysids were
then postfixed in a 2% Os0 4 solution for 1 h prior to dehydration in a graded alcohol series:
50 % for 30 min, 70 % for 30 min, 90 % for
30 min, followed by three washings in 100% alcohol for 30 min each. The procedure was the
same for ostracods except that they were postfixed in a 1% Os0 4 solution and then dehydrated. For chaetognaths, specimens were dehydrated only in the above-mentioned graded alcohol
series. All specimens were then critica! pointdried with liquid C0 2 for 3-5 h. C0 2 was
changed every hour. Specimens were then slowly
brought to a tempera ture of 50 oc and a pressure
of99 atm (1450 psi). The pressure was gradually
brought back to normal within 30 min and specimens were slowly cooled to ambient temperature. The specimens were then mounted on stubs
with adhesive tape and coated with gold for 3
min at a setting of 2.2 k V and 1 O mA of the sputtering apparatus.