Organics
229
between quantity of adenosine triphosphate and other parameters for the evaluation
of biomass is reviewed.
9.4.9
Organosu/phur Compounds
Beehard and Rayburn [662] determined volatile organic sulphides in fresh water algae.
Andreae [663] has described a gas chromatographic method for the determination
of nanogram quantities of dimethyl sulphoxide in phytoplankton. The method involves a chemical reduction to dimethyl sulphide with chromium(II) on sodium borohydride, which is then determined gas chromatographically using a flame photometric
detector.
Andreae [663] investigated two different apparatus configurations. One consisted
of a reaction/trapping apparatus connected by a six-way valve to a gas chromatograph equipped with a flame ionization detector, and the other combined the trapping
and separation functions in one column, which was attached to a flame photometric
detector. The gas chromatographic flame ionization detector system was identical to
that described by Andreae [664] for the analysis of methylarsenicals, with the exception that a reaction vessel which allowed the injection of solid sodium borohydride
pellets was used.
Andreae tested a large number of sulphur compounds in order to investigate potential
positive interferences due to the formation of dimethylsulphide from the reaction of
sulphur compounds, other than dimethyl sulphoxide, to form dimethyl sulphide. The
only compound other than dimethyl sulphoxide which gave a positive reaction with
sodium borohydride was dimethylpropiothetin «CH3)2S+CH2CH2COO-), an organosulphur compound occurring in some algae.
Andreae [664] showed that dimethyl sulphoxide is a common constituent in natural
waters. Its occurrence in sea water is restricted to the zone of light penetration. This
fact, and the abundance of dimethyl sulphoxide in the medium after the growth of
phytoplankton, suggest that it occurs as an end-product of algal metabolism.
9.4.70
Anatoxin a
High performance liquid chromatography combined with ultraviolet spectroscopy
has been used to determine this toxin in algae [665]. A temperature of 55°C is
recommended, either in a normal phase silica-A column, using 80 % isopropane in
hexane, or a reverse phase CIS column, using an aqueous solution of acetonitrile. The
toxin was separated from other components in aqueous resuspensions of dried extracts of algal cultures.
9.4.11
Uronic Acids and A/doses
Walters and Hedges [763] carried out a simultaneous determination uronic and aldoses
in plankton and plant tissues by capillary gas chromatography, following conver-
229
between quantity of adenosine triphosphate and other parameters for the evaluation
of biomass is reviewed.
9.4.9
Organosu/phur Compounds
Beehard and Rayburn [662] determined volatile organic sulphides in fresh water algae.
Andreae [663] has described a gas chromatographic method for the determination
of nanogram quantities of dimethyl sulphoxide in phytoplankton. The method involves a chemical reduction to dimethyl sulphide with chromium(II) on sodium borohydride, which is then determined gas chromatographically using a flame photometric
detector.
Andreae [663] investigated two different apparatus configurations. One consisted
of a reaction/trapping apparatus connected by a six-way valve to a gas chromatograph equipped with a flame ionization detector, and the other combined the trapping
and separation functions in one column, which was attached to a flame photometric
detector. The gas chromatographic flame ionization detector system was identical to
that described by Andreae [664] for the analysis of methylarsenicals, with the exception that a reaction vessel which allowed the injection of solid sodium borohydride
pellets was used.
Andreae tested a large number of sulphur compounds in order to investigate potential
positive interferences due to the formation of dimethylsulphide from the reaction of
sulphur compounds, other than dimethyl sulphoxide, to form dimethyl sulphide. The
only compound other than dimethyl sulphoxide which gave a positive reaction with
sodium borohydride was dimethylpropiothetin «CH3)2S+CH2CH2COO-), an organosulphur compound occurring in some algae.
Andreae [664] showed that dimethyl sulphoxide is a common constituent in natural
waters. Its occurrence in sea water is restricted to the zone of light penetration. This
fact, and the abundance of dimethyl sulphoxide in the medium after the growth of
phytoplankton, suggest that it occurs as an end-product of algal metabolism.
9.4.70
Anatoxin a
High performance liquid chromatography combined with ultraviolet spectroscopy
has been used to determine this toxin in algae [665]. A temperature of 55°C is
recommended, either in a normal phase silica-A column, using 80 % isopropane in
hexane, or a reverse phase CIS column, using an aqueous solution of acetonitrile. The
toxin was separated from other components in aqueous resuspensions of dried extracts of algal cultures.
9.4.11
Uronic Acids and A/doses
Walters and Hedges [763] carried out a simultaneous determination uronic and aldoses
in plankton and plant tissues by capillary gas chromatography, following conver-