460
showed greater TH-ir fi ber density within the OE nucleus, and more specifi cally, a
greater percentage of CHAT-ir defi ned somata and dendritic area contacted by TH-ir
fi bers (Fig. 10 ). Interestingly, the seasonal pattern seen in the saccule was opposite
to that found in the OE: winter, non-reproductive females had a greater number of
TH-ir puncta as well as a greater area per TH-ir punctum, while no signifi cant differences were found between seasons with regard to ChAT-ir puncta (Fig. 11 ). Since
TH-ir neurons in TPp are known to project to several auditory nuclei as well as the
OE and saccule, we also quantifi ed this cell group between summer and winter
females. While we did not fi nd differences in number of TH-ir neurons, we did
Fig. 8 Seasonal differences in TH-ir fi ber density in forebrain auditory nuclei. ( a , b ) Central posterior nucleus of the thalamus (CP) and ( d , e ) lateral division of nucleus preglomerulosus (PGl).
Left edge in a and b is midline of brain. Left edge in d and e is lateral edge of brain. Data in c and
f are represented as percent area of the nucleus that contains TH-ir (mean ± SE). * p = 0.03;
*** p = 0.0003. Scale bar = 100 μm. From Forlano et al. ( 2015a )
P.M. Forlano and J.A. Sisneros
showed greater TH-ir fi ber density within the OE nucleus, and more specifi cally, a
greater percentage of CHAT-ir defi ned somata and dendritic area contacted by TH-ir
fi bers (Fig. 10 ). Interestingly, the seasonal pattern seen in the saccule was opposite
to that found in the OE: winter, non-reproductive females had a greater number of
TH-ir puncta as well as a greater area per TH-ir punctum, while no signifi cant differences were found between seasons with regard to ChAT-ir puncta (Fig. 11 ). Since
TH-ir neurons in TPp are known to project to several auditory nuclei as well as the
OE and saccule, we also quantifi ed this cell group between summer and winter
females. While we did not fi nd differences in number of TH-ir neurons, we did
Fig. 8 Seasonal differences in TH-ir fi ber density in forebrain auditory nuclei. ( a , b ) Central posterior nucleus of the thalamus (CP) and ( d , e ) lateral division of nucleus preglomerulosus (PGl).
Left edge in a and b is midline of brain. Left edge in d and e is lateral edge of brain. Data in c and
f are represented as percent area of the nucleus that contains TH-ir (mean ± SE). * p = 0.03;
*** p = 0.0003. Scale bar = 100 μm. From Forlano et al. ( 2015a )
P.M. Forlano and J.A. Sisneros