3 FISH on Chromosome Preparations of Peripheral Blood
81
case some cross-hybridization problems may arise. The incubation
time has to be tested and adjusted according to the probe type.
10. During the washing steps, it is important to prevent the slide surfaces
drying out, otherwise background problems may arise.
Acknowledgements. The continuos support of the Carl Zeiss GmbH (Jena, Germany) is
gratefully acknowledged.
References
Claussen, U, Lemke, J, Michel, S, Chudoba, I, Miihlig, P, Westermann, M, Claussen, J,
Rubtsov, N, Grummt U-W (1999) The concept of chromosome condensation and
de-condensation during mitosis has to be replaced by the concept of chromosome
region specific swelling. Cytogenet Cell Genet 1999; Vol 85: p 166
Ford, CE, Hamerton, JL (1956) Ford, CE, Hamerton, JL (1956) The chromosomes in
man. Nature 178:1020-1030
Heng, HHQ, Tsui L-C (1993) Modes of DAPI banding and simultaneous in situ hybridization. Chromosoma, 102:325-332
Hliscs, R, Miihlig, P, Claussen, U (1997) The spreading of metaphases is a slow process
which leads to a stretching of chromosomes. Cytogenet Cell Genet, 76:167-171
Hsu TC (1952) Mammalian chromosomes in vitro. 1. karyotype of man. J of Heredity,
43:167-172
Hughes A (1952) Some effects of abnormal toxicity on dividing cells in chick tissue
cultures. Quart J of Mikroscop Sci, 93:207
Moorhead, PS, Nowell, PC, Mellman, WJ (1960) Chromosome preparations of leukocytes cultured from human peripheral blood. Exp Cell Res 20: 613-616
Nietzel A, Rocchi M, Heller A, Starke H, Wlodarska I, Beensen V, Claussen U, Liehr 7
(2001) A new multicolor-FISH approach for the characterization of marker chromosomes: Centromere-specific multicolor-FISH (cenM-FISH). Hum Genet, 108:
199-204
Nowell, PC (1960) Phytohemagglutinin: An initiator of mitosis in cultures of normal
human leukocytes. Cancer Res 20:462-466
Senger, G, Chudoba, I, Plesch A (1998) Multicolor-FISH - the identification of chromosome aberrations by 24 colors. BIOforum, 9/98: 99-503
Spurbeck, JL, Zinsmeister, AR, Meyer, KJ, Jatal, SM (1996) Dynamics of chromosome
spreading. Am J Med Genet, 61:387-393
Tjio, JH, Levan, A (1956) The chromosome number of man. Hereditas, 42:6-9
Wegner, RD (1999) Diagnostic Cytogenetics. Springer, Berlin Heidelberg
81
case some cross-hybridization problems may arise. The incubation
time has to be tested and adjusted according to the probe type.
10. During the washing steps, it is important to prevent the slide surfaces
drying out, otherwise background problems may arise.
Acknowledgements. The continuos support of the Carl Zeiss GmbH (Jena, Germany) is
gratefully acknowledged.
References
Claussen, U, Lemke, J, Michel, S, Chudoba, I, Miihlig, P, Westermann, M, Claussen, J,
Rubtsov, N, Grummt U-W (1999) The concept of chromosome condensation and
de-condensation during mitosis has to be replaced by the concept of chromosome
region specific swelling. Cytogenet Cell Genet 1999; Vol 85: p 166
Ford, CE, Hamerton, JL (1956) Ford, CE, Hamerton, JL (1956) The chromosomes in
man. Nature 178:1020-1030
Heng, HHQ, Tsui L-C (1993) Modes of DAPI banding and simultaneous in situ hybridization. Chromosoma, 102:325-332
Hliscs, R, Miihlig, P, Claussen, U (1997) The spreading of metaphases is a slow process
which leads to a stretching of chromosomes. Cytogenet Cell Genet, 76:167-171
Hsu TC (1952) Mammalian chromosomes in vitro. 1. karyotype of man. J of Heredity,
43:167-172
Hughes A (1952) Some effects of abnormal toxicity on dividing cells in chick tissue
cultures. Quart J of Mikroscop Sci, 93:207
Moorhead, PS, Nowell, PC, Mellman, WJ (1960) Chromosome preparations of leukocytes cultured from human peripheral blood. Exp Cell Res 20: 613-616
Nietzel A, Rocchi M, Heller A, Starke H, Wlodarska I, Beensen V, Claussen U, Liehr 7
(2001) A new multicolor-FISH approach for the characterization of marker chromosomes: Centromere-specific multicolor-FISH (cenM-FISH). Hum Genet, 108:
199-204
Nowell, PC (1960) Phytohemagglutinin: An initiator of mitosis in cultures of normal
human leukocytes. Cancer Res 20:462-466
Senger, G, Chudoba, I, Plesch A (1998) Multicolor-FISH - the identification of chromosome aberrations by 24 colors. BIOforum, 9/98: 99-503
Spurbeck, JL, Zinsmeister, AR, Meyer, KJ, Jatal, SM (1996) Dynamics of chromosome
spreading. Am J Med Genet, 61:387-393
Tjio, JH, Levan, A (1956) The chromosome number of man. Hereditas, 42:6-9
Wegner, RD (1999) Diagnostic Cytogenetics. Springer, Berlin Heidelberg