1 FISH Techniques, FISH Probes and Their Applications in Medicine and Biology - An Overview
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Fig. 5. Technical evolution in whole genome screening techniques for ascertainment ofkaryotype. A Metaphase spread
of unbanded chromosomes (Courtesey ofProf. G.B. Petersen) B Karyotype based on chromosome size before banding
techniques were available. C Q-banded metaphase spread showing a t(2;19). D Automated karyotyping with chromosome ideograms ofthe metaphase from C. EComparativegenomic hybridization analysis identifying a trisomy 18 (insert
shows 2 of the 24 computed red-green ratio profiles obtained by the analysis) - chromosome 18 (green arrows) and
chromosome 2 (white arrows). F Spectral karyotyping identifying a supernumerary marker chromosome (gray arrow)
derived from chromosome 3. Details on experimental conditions can be obtained by contacting the authors.